Rabbit CENPU / MLF1IP Polyclonal Antibody | anti-CENPU antibody

Rabbit Polyclonal to Human CENPU / MLF1IP

Cat. Num. AAA240746

• Gene Names: CENPU; KLIP1; PBIP1; CENP50; MLF1IP; CENPU50
• Reactivity: Bovine, Chimpanzee, Dog, Human
• Applications: Immunohistochemistry, Western Blot, Immunoprecipitation, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CENPU / MLF1IP; Polyclonal Antibody; Rabbit Polyclonal to Human CENPU / MLF1IP; Anti-CENPU / MLF1IP Antibody (phospho-Thr78) IHC-plus; CENPU; CENP-U; CENP50; CENPU50; Centromere protein U; ICEN24; KLIP1; Polo-box-interacting protein 1; MLF1-interacting protein; CENP-50; Centromere protein of 50 kDa; MLF1 interacting protein; MLF1IP; PBIP1; Human CENPU; anti-CENPU antibody

• Host: Rabbit
• Reactivity: Bovine, Chimpanzee, Dog, Human
• Clonality: Polyclonal
• Specificity: Amino acids surrounding Thr78 of human MLF1IP protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 418

• Applicable Applications for anti-CENPU antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: ELISA (1:5000 - 1:25000), IHC-P (10 ug/ml), IP, WB (1:500 - 1:2000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human MLF1IP / PBIP1.
• Immunogen Type: Synthetic peptide
• Immunogen: CENPU / MLF1IP antibody was raised against synthetic peptide from human MLF1IP / PBIP1.
• Antigen Modification: pThr78
• Preparation and Storage: Store vial at -20 degree C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MLF1IP / PBIP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Anti-MLF1IP Antibody - Western Blot. Western blot of affinity purified anti-MLF1IP antibody shows detection of MLF1IP (arrowhead) in HeLa cells transfected with ZZ-tagged MLF1IP (Lane 1). Lane 2 is lysate from non-transfected HeLa cells, and Lane 3 is lysate from HeLa cells containing a knock-out mutation for PBIP1/MLF1IP. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.)

Related Product Information for anti-CENPU antibody

Myeloid leukemia factor-1 (MLF1) Interacting protein, also known as PBIP1, is a novel gene that encodes a putative transcriptional repressor. It is localized to human chromosome 4q35.1, is expressed in both the nuclei and cytoplasm of cells, and is a novel substrate for polo-like kinase (PLK1). Phosphorylation by PLK1 regulates the PLK1-MLF1 interaction and localization to the kinetochores promoting proper chromosome segregation.

NCBI and Uniprot Product Information

• NCBI GI #: 38016935
• NCBI GeneID: 79682
• NCBI Accession #: NP_078905.2
• NCBI GenBank Nucleotide #: NM_024629.3
• UniProt Accession #: Q71F23; Q09GN2; Q32Q71; Q9H5G1; A2RRD9
• Molecular Weight: 38,102 Da
• NCBI Official Full Name: centromere protein U
• NCBI Official Synonym Full Names: centromere protein U
• NCBI Official Symbol: CENPU
• NCBI Official Synonym Symbols: KLIP1; PBIP1; CENP50; MLF1IP; CENPU50
• NCBI Protein Information: centromere protein U; MLF1 interacting protein; MLF1-interacting protein; centromere protein of 50 kDa; polo-box-interacting protein 1; interphase centromere complex protein 24; KSHV latent nuclear antigen interacting protein 1; KSHV latent nuclear antigen-interacting protein 1
• UniProt Protein Name: Centromere protein U
• Protein Family: Centromere protein
• UniProt Gene Name: CENPU
• UniProt Synonym Gene Names: ICEN24; KLIP1; MLF1IP; PBIP1; CENP-U; CENP-50
• UniProt Entry Name: CENPU_HUMAN

NCBI Description

The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). MLF1IP, or CENPU, is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM, Mar 2008]

Uniprot Description

CENPU: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 4q35.1

Cellular Component: nucleoplasm; microtubule organizing center; nucleus; cytosol

Biological Process: nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; chordate embryonic development; viral reproduction; transcription, DNA-dependent; regulation of transcription, DNA-dependent; mitotic cell cycle