Testing Data
Mouse anti-Mouse, Rat LEFTY A Monoclonal Antibody
Anti-Human LEFTY A Antibody
Reactivity
Mouse, Rat
Applications
ELISA, Western Blot
Purity
Protein A Purified
Synonyms
LEFTY A; Monoclonal Antibody; Anti-Human LEFTY A Antibody; Human LEFTY A; anti-LEFTY A antibody
Host
Mouse
Reactivity
Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Specificity
This protein A purified mouse monoclonal antibody reacts with a 30 kDa protein corresponding to human LEFTY. No reactivity occurs against 6X HIS tag. Significant sequence homology exists between the human and mouse forms of LEFTY. Cross reactivity is expected with mouse and rat forms of the protein. Reactivity with other sources has not been determined.
Purity/Purification
Protein A Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-LEFTY A antibody
ELISA (EIA), Western Blot (WB)
Application Notes
This antibody is suitable for ELISA and western blotting. The antibody may be used for other applications, such as RIA, immunohistochemistry or immunoprecipitation, but specific reaction conditions have not been developed.
Immunogen
A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-LEFTY A antibody
During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-beta family, including gene products_recover derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-beta-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.
Similar Products
Product Notes
The LEFTY A (Catalog #AAA4156812) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Human LEFTY An Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LEFTY A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). This antibody is suitable for ELISA and western blotting. The antibody may be used for other applications, such as RIA, immunohistochemistry or immunoprecipitation, but specific reaction conditions have not been developed. Researchers should empirically determine the suitability of the LEFTY A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LEFTY A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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