Western Blot (WB)
Mouse Nop1p Antibody | anti-Nop1p antibody
Nop1p Antibody
Western Blot (WB)
Western Blot (WB)
NCBI and Uniprot Product Information
Uniprot Description
Function: Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Involved in the biogenesis of the 18S rRNA. Ref.1 Ref.5
Subunit structure: Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain SNU13, NOP1, SIK1/NOP56 and NOP58, plus a guide RNA. Interacts with snoRNA U3. Interacts with MPP10, NOP58, SIK1 and SOF1. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.1 Ref.6 Ref.7 Ref.8
Subcellular location: Nucleus › nucleolus. Note: Fibrillar region of the nucleolus. Ref.1 Ref.2
Post-translational modification: Methylated by HMT1, forming asymmetric dimethylarginines (DMA) within a domain referred to as an RGG box, made up of repeated Gly-Gly dipeptides interspersed with Arg and aromatic residues.
Sequence similarities: Belongs to the methyltransferase superfamily. Fibrillarin family.
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Product Notes
The Nop1p nop1 (Catalog #AAA415392) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Nop1p can be used in a range of immunoassay formats including, but not limited to, ECL, Western Blot (WB), Immunofluorescence (IF). Western blots of yeast protein samples: 1:5,000 (cell lysates) to 1:12,500 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) western detection methods: 1:500 to 1:2,500. For immunofluorescence on yeast cells: 1:1,000 to 1: 10,000. Researchers should empirically determine the suitability of the Nop1p nop1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nop1p, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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