Immunofluorescence (IF)
(SH-SY5Y cells stained with Fibrillarin antibody, showing prominent specular nucleolar staining)
Mouse NOP1 Monoclonal Antibody | anti-NOP1 antibody
NOP1 antibody
Reactivity
Specific for the ~34k fibrilarin protein in Western blots of human, rat, plant, Drosophila, C. elegans, and S. pombe. Expected to work on other mammal tissues.
Applications
Western Blot, Immunofluorescence
Synonyms
NOP1; Monoclonal Antibody; NOP1 antibody; Monoclonal NOP1; Anti-NOP1; NOP 1; NOP-1; Nucleolar protein 1; anti-NOP1 antibody
Host
Mouse
Reactivity
Specific for the ~34k fibrilarin protein in Western blots of human, rat, plant, Drosophila, C. elegans, and S. pombe. Expected to work on other mammal tissues.
Clonality
Monoclonal
Isotype
IgG1
Clone Number
38F3
Form/Format
Supplied in liquid form in 10 mM NaN3
Sequence Length
154
Applicable Applications for anti-NOP1 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:1000
IF: 1:500
IF: 1:500
Biological Significance
Nop1 is a phylogenetically conserved protein essential for efficient processing of pre-rRNA through its association with a class of small nucleolar RNAs during ribosomal biogenesis. Small nucleolar RNAs (snoRNAs) are associated in ribonucleoprotein particles localized to the nucleolus (snoRNPs). Nop1 is structurally and functionally homologous to vertebrate fibrillarin and is essential for viability.
Preparation and Storage
Upon receipt store at -20°C.
Immunofluorescence (IF)
(SH-SY5Y cells stained with Fibrillarin antibody, showing prominent specular nucleolar staining)
Immunofluorescence (IF)
(SH-SY5Y cells stained with Fibrillarin antibody, showing prominent specular nucleolar staining)
Western Blot (WB)
(Western blot of HeLa lysate showing specific immunolabeling of ~ 34k fibrillarin protein)
Western Blot (WB)
(Western blot of HeLa lysate showing specific immunolabeling of ~ 34k fibrillarin protein)
Related Product Information for anti-NOP1 antibody
Mouse monoclonal NOP1 antibody
Product Categories/Family for anti-NOP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
NOP1, partial
UniProt Protein Name
rRNA 2'-O-methyltransferase fibrillarin
UniProt Gene Name
NOP1
UniProt Synonym Gene Names
LOT3; Nucleolar protein 1; U3 snoRNA-associated protein NOP1
UniProt Entry Name
FBRL_YEAST
Uniprot Description
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed:1825809). Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Involved in the biogenesis of the 18S rRNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).
Similar Products
Product Notes
The NOP1 nop1 (Catalog #AAA832027) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NOP1 antibody reacts with Specific for the ~34k fibrilarin protein in Western blots of human, rat, plant, Drosophila, C. elegans, and S. pombe. Expected to work on other mammal tissues. and may cross-react with other species as described in the data sheet. AAA Biotech's NOP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:1000 IF: 1:500. Researchers should empirically determine the suitability of the NOP1 nop1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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