Rabbit anti-Human, Mouse Fibrillarin Polyclonal Antibody | anti-FBL antibody

Fibrillarin Polyclonal Antibody

Cat. Num. AAA3012695

• Gene Names: FBL; FIB; FLRN; Nop1; RNU3IP1
• Reactivity: Human, Mouse
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Peptide affinity purified.
• Synonyms: Fibrillarin; Polyclonal Antibody; Fibrillarin Polyclonal Antibody; FBL; FIB1; FLRN; 34 kD nucleolar scleroderma antigen; 34 kDa nucleolar scleroderma antigen; Fbl; FBRL; FIB; Histone-glutamine methyltransferase; Nop1p; RNA U3 small nucleolar interacting protein 1; RNU3IP1; rRNA 2' O methyltransferase fibrillarin; rRNA 2'-O-methyltransferase fibrillarin; anti-FBL antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Fibrillarin polyclonal antibody detects endogenous levels of Fibrillarin protein.
• Purity/Purification: Peptide affinity purified.
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 321

• Applicable Applications for anti-FBL antibody: Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:1,000; FC/FACS: 1:50-1:100
• Immunogen: Synthetic peptide of C-terminal human Fibrillarin.
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Fibrillarin on different cell lysates using anti-Fibrillarin antibody at 1/1,000 dilution. Positive control: Lane1: Hela Lane2: HepG2Lane3: 293T Lane4: NIH-3T3)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HL-60 cells with Fibrillarin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

Related Product Information for anti-FBL antibody

Fibrillarin is a widely occurring, basic, nonhistone protein that is localized exclusively in the fibrillar region of the nucleolus, including both the dense fibrillar and the fibrillar center regions. Fibrillarin is also expressed in HeLa cells, 3T3 cells, and human peripheral blood lymphocytes. In metaphase and anaphase, fibrillarin is found on putative chromosomal nucleolar regions (NORs). During telophase, fibrillarin is an early marker for the site of the newly forming nucleolus. The structure of fibrillarin includes an RNA-binding domain and an RNP consensus sequence, which is consistent with the association of fibrillarin with the U3 small nucleolar RNA. Fibrillarin is involved in processing rRNA transcripts in the nucleolus.

NCBI and Uniprot Product Information

• NCBI GI #: 31395
• NCBI GeneID: 2091
• UniProt Accession #: P22087; P35550
• Molecular Weight: 34kDa
• NCBI Official Full Name: fibrillarin
• NCBI Official Synonym Full Names: fibrillarin
• NCBI Official Symbol: FBL
• NCBI Official Synonym Symbols: FIB; FLRN; Nop1; RNU3IP1
• NCBI Protein Information: rRNA 2'-O-methyltransferase fibrillarin
• UniProt Protein Name: rRNA 2'-O-methyltransferase fibrillarin
• Protein Family: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase
• UniProt Gene Name: FBL
• UniProt Synonym Gene Names: FIB1; FLRN
• UniProt Entry Name: FBRL_HUMAN

NCBI Description

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]

Uniprot Description

FBL: Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'- hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Interacts with NOLC1. Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain NHP2L1, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs. Component of several ribosomal and nucleolar protein complexes. Interacts with PRMT5 and UTP20. Interacts with DDX5. Belongs to the methyltransferase superfamily. Fibrillarin family.

Protein type: RNA-binding; RNA processing; Methyltransferase; Nucleolus; EC 2.1.1.-

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: Cajal body; granular component; membrane; nucleolus; nucleus

Molecular Function: protein binding; RNA binding

Biological Process: osteoblast differentiation; snoRNA metabolic process; tRNA processing; rRNA processing