Mouse ALF Monoclonal Antibody | anti-ALF antibody

Anti-ALF Antibody

Cat. Num. AAA415065

• Gene Names: GTF2A1L; ALF
• Applications: ELISA, Western Blot
• Purity: Affinity purified
• Synonyms: ALF; Monoclonal Antibody; Anti-ALF Antibody; anti-ALF antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Anti-ALF recognizes recombinant protein ALF
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 1668

• Applicable Applications for anti-ALF antibody: ELISA (EIA), Western Blot (WB-Re)
• Application Notes: WB: 1:1000 analysis of recombinant protein ALF with Anti-ALF
• Raised In: Purified mouse monoclonal antibody generated using E.coli expressed recombinant protein ALF as immunogen.
• Storage Buffer: citrate-tris-HCl buffer pH7.0 with 0.02% Proclin 300.
• Immunogen: Recombinant protein corresponding to full length ALF.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

References

None

NCBI and Uniprot Product Information

• NCBI GI #: 26787967
• NCBI GeneID: 11036
• NCBI Accession #: NP_006863.2
• NCBI GenBank Nucleotide #: NM_006872.2
• Molecular Weight: 48,504 Da
• NCBI Official Full Name: Homo sapiens general transcription factor IIA, 1-like (GTF2A1L), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: general transcription factor IIA, 1-like
• NCBI Official Symbol: GTF2A1L
• NCBI Official Synonym Symbols: ALF
• NCBI Protein Information: TFIIA-alpha and beta-like factor; GTF2A1-like factor; TFIIA large subunit isoform ALF; TFIIA-alpha/beta-like factor; general transcription factor II A, 1-like factor
• UniProt Protein Name: TFIIA-alpha and beta-like factor
• Protein Family: Protein
• UniProt Gene Name: GTF2A1L
• UniProt Entry Name: TF2AY_HUMAN

NCBI Description

The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of transcription factor IIA (TFIIA) on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology. Alternative splicing for this locus has been observed and two variants, encoding distinct isoforms, have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Mar 2014]

Uniprot Description

GTF2A1L: May function as a testis specific transcription factor. Binds DNA in conjunction with GTF2A2 and TBP (the TATA-binding protein) and together with GTF2A2, allows mRNA transcription. Belongs to the TFIIA subunit 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 2p16.3

Cellular Component: cytoplasm; transcription factor TFIIA complex

Molecular Function: DNA binding; transcription coactivator activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; cognition