DCC-interacting Protein 13-beta Recombinant Protein | APPL2 recombinant protein
DCC-interacting Protein 13-beta
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
>95%; SDS PAGE to determine purity of the protein
Synonyms
DCC-interacting Protein 13-beta; DIP13B; FLJ10659; Recombinant APPL2 with His-tag (Human); APPL2 recombinant protein
Host
E Coli
Purity/Purification
>95%; SDS PAGE to determine purity of the protein
Form/Format
Lyophilized in 1mg/mL in PBS
Sequence
MSYYHHHHHH DYDIPTTENL YFQGAMGSGI QRPTSTPAVD KLLLEEALQD SPQTRSLLSV FEEDAGTLTD YTNQLLQAMQ RVYGAQNEMC LATQQLSKQL LAYEKQNFAL GKGDEEVIST LHYFSKVVDE LNLLHTELAK QLADTMVLPI IQFREKDLTE VSTLKDLFGL ASNEHDLSMA KYSRLPKKKE NEKVKTEVGK EVAAARRKQH LSSLQYYCAL NALQYRKQMA MMEPMIGFAH GQINFFKKGA EMFSKRMDSF LSSVADMVQS IQVELEAEAE KMRVSQQELL SVDESVYTPD SDVAAPQINR NLIQKAGYLN LRNKTGLVTT TWERLYFFTQ GGNLMCQPRG AVAGGLIQDL DNCSVMAVDC EDRRYCFQIT TPNGKSGIIL QAESRKENEE WICAINNISR QIYLTDNPEA VAIKLNQTAL QAVTPITSFG KKQESSCPSQ NLKNSEMENE NDKIVPKATA SLPEAEELIAPGTPIQFDIV LPATEFLDQN RGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARA IHNIFRMTES HLMVTSQSLR LIDPQTQVSR ANFELTSVTQ FAAHQENKRL VGFVIRVPES TGEESLSTYI FESNSEGEKI CYAINLGKEI IEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA
Sequence Length
664
Applicable Applications for APPL2 recombinant protein
ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
Species
Human
Tag
His Tag
Reconstitution
Add deionized water to prepare a working stock solution of approximately 1mg/mL and let the lyophilized pellet dissolve completely.
Quality Control Test
BCA to determine quantity of the protein.
Preparation and Storage
Store lyophilized protein at -20 degree C. Aliquot reconstituted protein and store at -80 degree C. Avoid repeated freezing /thawing cycles.
Related Product Information for APPL2 recombinant protein
APPL2, also termed as Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2, has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, APPL1, adiponectin receptors, and proteins of the NuRD/MeCP1 complex.
Total 699AA. Mw: 78.5 kDa (calculated). N-terminal His-tag and TEV cleavage site, 36 extra AA.
Total 699AA. Mw: 78.5 kDa (calculated). N-terminal His-tag and TEV cleavage site, 36 extra AA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
78.5kDa
NCBI Official Full Name
DCC-interacting protein 13-beta isoform 1
UniProt Protein Name
DCC-interacting protein 13-beta
Protein Family
UniProt Gene Name
APPL2
UniProt Synonym Gene Names
DIP13B; Dip13-beta
UniProt Entry Name
DP13B_HUMAN
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Product Notes
The APPL2 appl2 (Catalog #AAA398076) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DCC-interacting Protein 13-beta can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the APPL2 appl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSYYHHHHHH DYDIPTTENL YFQGAMGSGI QRPTSTPAVD KLLLEEALQD SPQTRSLLSV FEEDAGTLTD YTNQLLQAMQ RVYGAQNEMC LATQQLSKQL LAYEKQNFAL GKGDEEVIST LHYFSKVVDE LNLLHTELAK QLADTMVLPI IQFREKDLTE VSTLKDLFGL ASNEHDLSMA KYSRLPKKKE NEKVKTEVGK EVAAARRKQH LSSLQYYCAL NALQYRKQMA MMEPMIGFAH GQINFFKKGA EMFSKRMDSF LSSVADMVQS IQVELEAEAE KMRVSQQELL SVDESVYTPD SDVAAPQINR NLIQKAGYLN LRNKTGLVTT TWERLYFFTQ GGNLMCQPRG AVAGGLIQDL DNCSVMAVDC EDRRYCFQIT TPNGKSGIIL QAESRKENEE WICAINNISR QIYLTDNPEA VAIKLNQTAL QAVTPITSFG KKQESSCPSQ NLKNSEMENE NDKIVPKATA SLPEAEELIA PGTPIQFDIV LPATEFLDQN RGSRRTNPFG ETEDESFPEA EDSLLQQMFI VRFLGSMAVK TDSTTEVIYE AMRQVLAARA IHNIFRMTES HLMVTSQSLR LIDPQTQVSR ANFELTSVTQ FAAHQENKRL VGFVIRVPES TGEESLSTYI FESNSEGEKI CYAINLGKEI IEVQKDPEAL AQLMLSIPLT NDGKYVLLND QPDDDDGNPN EHRGAESEA. It is sometimes possible for the material contained within the vial of "DCC-interacting Protein 13-beta, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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