Western Blot (WB)
(Western blot analysis of APPL2 in20ug HEK293 (Lane 1) and MDAMB-231 (Lane 2) cell lysate usinganti-APPL2 followed by goat antirabbit antibody.)
Rabbit anti-Human Adaptor Protein, Phosphotyrosine Interacting with PH Domain And Leucine Zipper 2 (Appl2) Polyclonal Antibody | anti-APPL2 antibody
Adaptor Protein, Phosphotyrosine Interacting with PH Domain And Leucine Zipper 2 (Appl2): Human, rabbit polyclonal (Antibody)
Reactivity
Human
Applications
Western Blot, ELISA, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
Purity
Rabbit antiserum was purified by affinity APPL2 coupled column
Synonyms
Adaptor Protein; Phosphotyrosine Interacting with PH Domain And Leucine Zipper 2 (Appl2); Polyclonal Antibody; Phosphotyrosine Interacting with PH Domain And Leucine Zipper 2 (Appl2): Human; rabbit polyclonal (Antibody); Polyclonal Antibody against APPL2; anti-APPL2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects many types of APPL2 in different speciessuch as human, monkey, mouse, rat, etc (about 75kDa).
Purity/Purification
Rabbit antiserum was purified by affinity APPL2 coupled column
Form/Format
Solution in PBS
Sequence Length
664
Applicable Applications for anti-APPL2 antibody
Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
WB: This antibody can be used at 0.2 -0.3ug/mL with the appropriate secondary reagentsto detect APPL2.
Immunostaining-This antibody can be used at 2-3ug/mL with the appropriate secondary reagents todetect APPL2.
ELISA: This antibody can be used at 1.0-2.0ug/mL with the appropriate secondary reagents todetect APPL2.
Immunostaining-This antibody can be used at 2-3ug/mL with the appropriate secondary reagents todetect APPL2.
ELISA: This antibody can be used at 1.0-2.0ug/mL with the appropriate secondary reagents todetect APPL2.
Immunogen
Recombinant full-length human APPL2 expressed in E Coli cells
Preparation and Storage
Store at -20 degree C. For long-term storage, aliquot and freeze at -70 degree C. Avoid repeated freeze/defrost cycles.
Western Blot (WB)
(Western blot analysis of APPL2 in20ug HEK293 (Lane 1) and MDAMB-231 (Lane 2) cell lysate usinganti-APPL2 followed by goat antirabbit antibody.)
Western Blot (WB)
(Western blot analysis of APPL2 in20ug HEK293 (Lane 1) and MDAMB-231 (Lane 2) cell lysate usinganti-APPL2 followed by goat antirabbit antibody.)
Immunofluorescence (IF)
(Immunostaining of APPL2 in C2C12cells using anti-APPL2 followed bygoat anti-rabbit antibody, visualizedby confocal microscopy. )
Immunofluorescence (IF)
(Immunostaining of APPL2 in C2C12cells using anti-APPL2 followed bygoat anti-rabbit antibody, visualizedby confocal microscopy. )
Related Product Information for anti-APPL2 antibody
The adaptor proteins APPL1 and APPL2 (Adaptor protein-containing an NH2-terminal Bin/Amphiphysin/Rvs (BAR) domain, a central Pleckstrin homology (PH) domain, Phosphotyrosine-binding (PTB) domain, and Leucine zipper motif), regulate adiponectin action and insulin-sensitivity in multiple insulin-responsive tissues.
APPL2, also termed as Adaptor protein, phosphotyrosine interaction, PH domain and leucinezipper containing 2, has been shown to be involved in the regulation of cell proliferation, andin the crosstalk between the adiponectin signalling and insulin signalling pathways. Theencoded protein binds many other proteins, including RAB5A, APPL1, adiponectin receptors, and proteins of the NuRD/MeCP1 complex.
APPL2, also termed as Adaptor protein, phosphotyrosine interaction, PH domain and leucinezipper containing 2, has been shown to be involved in the regulation of cell proliferation, andin the crosstalk between the adiponectin signalling and insulin signalling pathways. Theencoded protein binds many other proteins, including RAB5A, APPL1, adiponectin receptors, and proteins of the NuRD/MeCP1 complex.
References
Relevant recent publications: 1. Signaling mechanisms underlying the insulin-sensitizing effects of adiponectin. Cheng KK, Lam KS, Wang B, Xu A. Best Pract Res Clin Endocrinol Metab. 2014 28(1):3-13
2. APPL1: role in adiponectin signaling and beyond. Deepa SS, Dong LQ. Am J Physiol Endocrinol Metab. 2009 296(1):E22-36
2. APPL1: role in adiponectin signaling and beyond. Deepa SS, Dong LQ. Am J Physiol Endocrinol Metab. 2009 296(1):E22-36
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2
Protein Family
Similar Products
Product Notes
The APPL2 (Catalog #AAA398024) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Adaptor Protein, Phosphotyrosine Interacting with PH Domain And Leucine Zipper 2 (Appl2): Human, rabbit polyclonal (Antibody) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Adaptor Protein, Phosphotyrosine Interacting with PH Domain And Leucine Zipper 2 (Appl2) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC). WB: This antibody can be used at 0.2 -0.3ug/mL with the appropriate secondary reagentsto detect APPL2. Immunostaining-This antibody can be used at 2-3ug/mL with the appropriate secondary reagents todetect APPL2. ELISA: This antibody can be used at 1.0-2.0ug/mL with the appropriate secondary reagents todetect APPL2. Researchers should empirically determine the suitability of the APPL2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Adaptor Protein, Phosphotyrosine Interacting with PH Domain And Leucine Zipper 2 (Appl2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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