Human COVID 19 Nucleocapsid (NP) IgM Coronavirus ELISA Kit | COVID-19 elisa kit
SARS-CoV-2 Nucleocapsid (NP) IgM ELISA Kit
Reactivity
Human
Synonyms
COVID 19 Nucleocapsid (NP) IgM Coronavirus; SARS-CoV-2 Nucleocapsid (NP) IgM ELISA Kit; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Nucleocapsid; Nucleoprotein (NP); COVID-19 elisa kit
Reactivity
Human
Application Notes
CE Marking: European CE Certification
The Positive Control in the Kit is a Humanized anti-Nucleocapsid IgM Antibody
The Positive Control in the Kit is a Humanized anti-Nucleocapsid IgM Antibody
Samples
Human serum or plasma
Preparation and Storage
The kit should be stored at 2-8 degree C upon receipt.
Related Product Information for COVID-19 elisa kit
Intended Uses: SARS-CoV-2 NP IgM ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for the detection and qualitative measurement of IgM class antibodies against the nucleocapsid protein (NP) of SARS-CoV-2 virus in human blood.
Principle of the Assay: SARS-CoV-2 NP IgM ELISA kit is a two-step incubation immunoassay kit. Recombinant nucleocapsid protein (NP) of SARS-CoV-2 pre-coated onto the polystyrene microwell strips can specifically recognize anti-NP antibodies in human serum or plasma specimens. After a 1-hour incubation, anti-NP antibodies are captured by immobilized NP protein while the unbound components are washed away. Afterwards, a detection solution containing HRP- conjugated anti-human IgM is added for another 1-hour incubation, wherein HRP-conjugated anti-human IgM binds to the IgM class antibodies previously bound to NP protein on the plate. After removal of nonspecific bindings, a HRP substrate solution containing 3,3?,5,5?-Tetramethylbenzidine (TMB) is added, resulting in the formation of a blue color. Color reaction is stopped by 2M H2SO4, transforming the blue color to yellow signals, which is quantified by an absorbance microplate reader at 450nm. The color intensity is proportional to the amount of anti-NP IgM antibodies captured inside the wells.
Principle of the Assay: SARS-CoV-2 NP IgM ELISA kit is a two-step incubation immunoassay kit. Recombinant nucleocapsid protein (NP) of SARS-CoV-2 pre-coated onto the polystyrene microwell strips can specifically recognize anti-NP antibodies in human serum or plasma specimens. After a 1-hour incubation, anti-NP antibodies are captured by immobilized NP protein while the unbound components are washed away. Afterwards, a detection solution containing HRP- conjugated anti-human IgM is added for another 1-hour incubation, wherein HRP-conjugated anti-human IgM binds to the IgM class antibodies previously bound to NP protein on the plate. After removal of nonspecific bindings, a HRP substrate solution containing 3,3?,5,5?-Tetramethylbenzidine (TMB) is added, resulting in the formation of a blue color. Color reaction is stopped by 2M H2SO4, transforming the blue color to yellow signals, which is quantified by an absorbance microplate reader at 450nm. The color intensity is proportional to the amount of anti-NP IgM antibodies captured inside the wells.
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Product Notes
The Human COVID-19 (Catalog #AAA398006) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA398006 ELISA Kit recognizes Human COVID-19. CE Marking: European CE Certification The Positive Control in the Kit is a Humanized anti-Nucleocapsid IgM Antibody. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Nucleocapsid (NP) IgM Coronavirus, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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