Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Placenta-specific protein 1 (PLAC1) Standard Curve.)
Rat Placenta-Specific Protein 1 (PLAC1) ELISA Kit | PLAC1 elisa kit
Rat Placenta-Specific Protein 1 (PLAC1) ELISA Kit
Reactivity
Rat
Synonyms
Placenta-Specific Protein 1 (PLAC1); Rat Placenta-Specific Protein 1 (PLAC1) ELISA Kit; PLAC1; CT92; cancer/testis antigen 92; PLAC1 elisa kit
Reactivity
Rat
Specificity
Rat Placenta-specific protein 1 (PLAC1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PLAC1. No significant cross-reactivity or interference between Rat PLAC1 and analogues was observed.
Sequence Length
173
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Placenta-specific protein 1 (PLAC1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Placenta-specific protein 1 (PLAC1) Standard Curve.)
Related Product Information for PLAC1 elisa kit
Description: This Rat Placenta-specific protein 1 (PLAC1) ELISA Kit employs a two-site sandwich ELISA to quantitate PLAC1 in samples. An antibody specific for PLAC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLAC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLAC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLAC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Cocchia et al. (2000) isolated a novel gene, which they designated PLAC1, approximately 65 kb telomeric of the HPRT gene. The mouse homolog was also found to map near Hprt on the X chromosome. Human PLAC1 is transcribed in the telomeric to centromeric direction, opposite to the orientation of HPRT, and contains 3 predicted exons. It encodes a deduced 212-amino acid protein that shares 60% sequence identity with mouse Plac1. The human and mouse proteins contain a signal peptide and a ZP3 motif. Northern blot analysis detected placenta-restricted expression of a 1.7-kb PLAC1 mRNA transcript. In situ hybridization studies in mouse detected Plac1 expression from 7.5 to 14.5 days postcoitum in ectoplacental cone, giant cells, and labyrinthine trophoblasts.
Background: Cocchia et al. (2000) isolated a novel gene, which they designated PLAC1, approximately 65 kb telomeric of the HPRT gene. The mouse homolog was also found to map near Hprt on the X chromosome. Human PLAC1 is transcribed in the telomeric to centromeric direction, opposite to the orientation of HPRT, and contains 3 predicted exons. It encodes a deduced 212-amino acid protein that shares 60% sequence identity with mouse Plac1. The human and mouse proteins contain a signal peptide and a ZP3 motif. Northern blot analysis detected placenta-restricted expression of a 1.7-kb PLAC1 mRNA transcript. In situ hybridization studies in mouse detected Plac1 expression from 7.5 to 14.5 days postcoitum in ectoplacental cone, giant cells, and labyrinthine trophoblasts.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
placenta-specific protein 1
NCBI Official Synonym Full Names
placenta-specific protein 1-like
NCBI Official Symbol
LOC102550080
NCBI Protein Information
placenta-specific protein 1
UniProt Protein Name
Placenta-specific protein 1
Protein Family
UniProt Gene Name
Plac1
UniProt Entry Name
PLAC1_RAT
Uniprot Description
Function: May play a role in placental development By similarityManual assertion inferred from sequence similarityUniProtKB:Q9HBJ0
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Product Notes
The Rat PLAC1 plac1 (Catalog #AAA9712110) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712110 ELISA Kit recognizes Rat PLAC1. It is sometimes possible for the material contained within the vial of "Placenta-Specific Protein 1 (PLAC1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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