Western Blot (WB)
((0.5ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Histone Deacetylase 1 Polyclonal Antibody | anti-HDAC1 antibody
Goat anti-Histone Deacetylase 1 Antibody
Western Blot: Appox 55kDa band observed in Human Lymph Node and Thymus lysates and approx 70kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 55.1kDa according to NP_004955.2). The observed 70kDa band represents the sumoylated protein according to David et al, J Biol Chem.2002 Jun 28;277(26): 23658-63; PMID: 11960997. Recommended concentration: 0.5-2ug/ml.
Chromatin Immunoprecipitation: A 3.5-fold increased presence of H3 onto the Human P21 locus observed when comparing the precipitations using thhis item with non-specific goat IgG to bring down HDAC1 in a chromatin lysate from MCF7 cells.
Western Blot (WB)
((0.5ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Testing Data
(ChIP of 2ug with 1ug MCF7 chromatin using the Chromatrap spin column sonication kit (Protein G) measuring H3 enrichment onto the p21 locus.)
Testing Data
(ChIP of 2ug with 1ug MCF7 chromatin using the Chromatrap spin column sonication kit (Protein G) measuring H3 enrichment onto the p21 locus.)
NCBI and Uniprot Product Information
NCBI Description
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]
Uniprot Description
HDAC1: a transcriptional regulator of the histone deacetylase family, subfamily 1. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events.
Protein type: EC 3.5.1.98; Nuclear receptor co-regulator; Hydrolase
Chromosomal Location of Human Ortholog: 1p34
Cellular Component: chromatin; cytoplasm; cytosol; histone deacetylase complex; nuclear chromatin; nucleoplasm; nucleus; NuRD complex; protein complex; Sin3 complex
Molecular Function: deacetylase activity; enzyme binding; histone deacetylase activity; histone deacetylase binding; NAD-dependent histone deacetylase activity (H3-K14 specific); NF-kappaB binding; nucleosomal DNA binding; protein binding; protein deacetylase activity; protein N-terminus binding; transcription activator binding; transcription factor activity; transcription factor binding
Biological Process: ATP-dependent chromatin remodeling; blood coagulation; chromatin modification; chromatin remodeling; circadian regulation of gene expression; embryonic digit morphogenesis; epidermal cell differentiation; histone deacetylation; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; odontogenesis of dentine-containing teeth; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid deacetylation; transcription, DNA-dependent; viral reproduction
Research Articles on HDAC1
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Product Notes
The HDAC1 hdac1 (Catalog #AAA422617) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Histone Deacetylase 1 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's Histone Deacetylase 1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Chromatin Immunoprecipitation (ChIP). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Appox 55kDa band observed in Human Lymph Node and Thymus lysates and approx 70kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 55.1kDa according to NP_004955.2). The observed 70kDa band represents the sumoylated protein according to David et al, J Biol Chem.2002 Jun 28;277(26): 23658-63; PMID: 11960997. Recommended concentration: 0.5-2ug/ml. Chromatin Immunoprecipitation: A 3.5-fold increased presence of H3 onto the Human P21 locus observed when comparing the precipitations using thhis item with non-specific goat IgG to bring down HDAC1 in a chromatin lysate from MCF7 cells. Researchers should empirically determine the suitability of the HDAC1 hdac1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KPEAKGVKEE VK. It is sometimes possible for the material contained within the vial of "Histone Deacetylase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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