Rabbit Collagen Type VI Antibody | anti-COL6A1 antibody

Collagen Type VI, human

Cat. Num. AAA397140

• Gene Names: COL6A1; OPLL; BTHLM1; UCHMD1
• Reactivity: Human, Bovine, Porcine, Chicken, Murine, Rat tissue
• Applications: Immunohistochemistry, Immunofluorescence, ELISA, immunoassay, Radioimmunoassay, Immunoblot, Western Blot, Western Blot
• Purity: Affinity Purified
• Synonyms: Collagen Type VI; human; anti-COL6A1 antibody

• Host: Rabbit
• Reactivity: Human, Bovine, Porcine, Chicken, Murine, Rat tissue
• Purity/Purification: Affinity Purified
• Form/Format: Lyophilized from phosphate buffered solution; no BSA and preservative added.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1028

• Applicable Applications for anti-COL6A1 antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA), Radioimmunoassay (RIA), Immunoblotting (IB), Western Blot (WB)
• Application Notes: IFA: 1:80; IHC-P: 1:1000; ELISA: 1:200
• Immunogen: Purified collagen type VI from human placenta
• Positive Control: Human placenta
• Incubation Time: IHC(P) 60 min at RT or 2-8 degree C over night
• Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 degree C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Preparation and Storage: Store at -20 degree C.

Related Product Information for anti-COL6A1 antibody

Collagen type VI is found as 5-10 nm diameter microfibrils (periodicity 100 nm) e.g. in placenta, skin, cartilage and cornea. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type VI 100%, human collagen type I, II, III, IV and V <0.1%; human elastin and human laminin <0.1%; human fibronectin < 1% (RIA at 1:10.000 dilution).

References

1. Ronzière M.C., Ricard-Blum S., Tiollier J., Hartmann D.J., Garrone R., Herbage D. (1990) Comparative analysis of collagens solubilized from human foetal, and normal and osteoarthritic adult articular cartilage, with emphasis on type VI collagen. Biochim Biophys Acta. Apr 19;1038(2):222-30. 2. Silvester LM, Luck MR (1999) Distribution of extracellular matrix components in the developing ruminant corpus luteum: a wound repair hypothesis for luteinization. J Reprod Fertil. May;116(1):187-98.

NCBI and Uniprot Product Information

• NCBI GI #: 87196339
• NCBI GeneID: 1291
• NCBI Accession #: NP_001839.2
• NCBI GenBank Nucleotide #: NM_001848.2
• UniProt Accession #: P12109; O00117; O00118; Q14040; Q14041; Q16258; Q7Z645; Q9BSA8
• Molecular Weight: 108,529 Da
• NCBI Official Full Name: collagen alpha-1(VI) chain
• NCBI Official Synonym Full Names: collagen type VI alpha 1 chain
• NCBI Official Symbol: COL6A1
• NCBI Official Synonym Symbols: OPLL; BTHLM1; UCHMD1
• NCBI Protein Information: collagen alpha-1(VI) chain
• UniProt Protein Name: Collagen alpha-1(VI) chain
• Protein Family: Collagen
• UniProt Gene Name: COL6A1

NCBI Description

The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively. The protein encoded by this gene is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy. [provided by RefSeq, Jul 2008]

Uniprot Description

Collagen VI acts as a cell-binding protein.