Rabbit MRPL13 Polyclonal Antibody | anti-MRPL13 antibody

MRPL13 Polyclonal Antibody

Cat. Num. AAA3007426

• Gene Names: MRPL13; L13; L13A; L13mt; RPL13; RPML13
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >96% (by SDS-PAGE).
• Synonyms: MRPL13; Polyclonal Antibody; MRPL13 Polyclonal Antibody; 39S ribosomal protein L13; mitochondrial; L13mt; MRP-L13; anti-MRPL13 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MRPL13 polyclonal antibody detects endogenous levels of MRPL13 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >96% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 178

• Applicable Applications for anti-MRPL13 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 125-169 of Human MRPL13.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MRPL13 polyclonal antibody at 1:500 dilutionLane1:SK-OVCAR3 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug))

Immunoprecipitation (IP)

(Immunoprecipitation of HEK293T cell lysate using MRPL13 polyclonal antibody (Sepharose Bead Conjugate) (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) (lane 4 ). Lane 1 is 30% input. The Western Blot was probed using MRPL13 # MBS3007426. “up-arrow” (supernatant) ;“down-arrow (deposition))

Related Product Information for anti-MRPL13 antibody

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.

NCBI and Uniprot Product Information

• NCBI GI #: 14165270
• NCBI GeneID: 28998
• NCBI Accession #: NP_054797.2
• NCBI GenBank Nucleotide #: NP_054797.2
• UniProt Accession #: Q9BYD1
• Molecular Weight: ~ 20kDa
• NCBI Official Full Name: 39S ribosomal protein L13, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein L13
• NCBI Official Symbol: MRPL13
• NCBI Official Synonym Symbols: L13; L13A; L13mt; RPL13; RPML13
• NCBI Protein Information: 39S ribosomal protein L13, mitochondrial
• UniProt Protein Name: 39S ribosomal protein L13, mitochondrial
• Protein Family: 39S ribosomal protein
• UniProt Gene Name: MRPL13
• UniProt Synonym Gene Names: L13mt; MRP-L13
• UniProt Entry Name: RM13_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPL13: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]

Protein type: Translation; Mitochondrial; RNA-binding; Ribosomal

Chromosomal Location of Human Ortholog: 8q22.1-q22.3

Cellular Component: mitochondrial inner membrane; mitochondrial ribosome; mitochondrial large ribosomal subunit

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: mitochondrial translation; translation; organelle organization and biogenesis