Rap 2A recombinant protein

Rap 2A

Cat. Num. AAA396488

• Gene Names: RAP2A; KREV; RAP2; K-REV; RbBP-30
• Reactivity: Human
• Applications: Affinity Binding
• Synonyms: Rap 2A; Rap 2A Recombinant G-Protein; G protein; Rap 2A recombinant protein

• Reactivity: Human
• Form/Format: Provided in 50mM Tris (pH 7.5) solution containing 150 mM NaCl, 10 mM MgCl2, 1 mM DTT, 10% glycerol
• Sequence Length: 183

• Applicable Applications for Rap 2A recombinant protein: Affinity Binding
• Immunogen: Recombinant full length human Rap1A protein expressed in e.coli
• Preparation and Storage: Product should be stored at -70 degree C. Aliquot to avoid freeze/thaw cycles

Related Product Information for Rap 2A recombinant protein

Rap2B is a 21 kDa small GTP-binding protein that that exhibits 46% identity with Ras proteins and is similarly post-translationally modified by farnesyl and palmitate groups. It colocalizes with several markers of the Golgi complex, thus indicating that its site of function was distinct from that of Rap1A. In contrast to Rap1A, Rap2B protein does not interfere with Ras-induced transformation suggesting that the activity of Rap proteins goes beyond the regulation of Ras protein function. Ras proteins provide a crucial link in the intracellular signalling cascades. Ras proteins are members of the small G protein family. These proteins are characterized by a molecular weight between 20 and 30 kDa, the ability to bind GTP or GDP and a low intrinsic GTPase activity. These proteins relay signals from the plasma membrane activated by receptor tyrosine kinases to the nucleus via a network of serine/threonine kinases. Ras proteins belong to a family of approximately 40 mammalian and 60 non-mammalian proteins. Ras proteins were first discovered as the hyperactive products of mutant Ras genes which promote cancer by disrupting the normal controls on cell proliferation and differentiation; about 30% of human cancers have mutations in a Ras gene.

Product Categories/Family for Rap 2A recombinant protein

Recombinant Protein; G Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 10518344
• NCBI GeneID: 5911
• NCBI Accession #: NP_066361.1
• NCBI GenBank Nucleotide #: NM_021033.6
• UniProt Accession #: P10114; Q5JSC1; Q5JSC2; B2RCJ1
• Molecular Weight: 20,615 Da
• NCBI Official Full Name: ras-related protein Rap-2a
• NCBI Official Synonym Full Names: RAP2A, member of RAS oncogene family
• NCBI Official Symbol: RAP2A
• NCBI Official Synonym Symbols: KREV; RAP2; K-REV; RbBP-30
• NCBI Protein Information: ras-related protein Rap-2a; RAP2, member of RAS oncogene family (K-rev)
• UniProt Protein Name: Ras-related protein Rap-2a
• UniProt Gene Name: RAP2A
• UniProt Entry Name: RAP2A_HUMAN

Uniprot Description

Function: Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading. Ref.15 Ref.16 Ref.18 Ref.19 Ref.21 Ref.24

Enzyme regulation: Activated by the guanine nucleotide-exchange factors RAPGEF3 and RAPGEF4 in a cAMP-dependent manner. Nucleotide exchange is also specifically stimulated by RAPGEF5, RASGEF1A and RASGEF1B.

Subunit structure: Interacts (GTP-bound form) with RUNDC3A. Interacts with RGS14; the interaction is GTP-dependent

By similarity. Interacts with PLCE1. Interacts with ARHGAP29, SGSM1, SGSM2 and SGSM3. Interacts (GTP-bound form preferentially) with TNIK (via the CNH domain); the interaction is direct and recruits RAP2A to the E3 ubiquitin ligase NEDD4. Interacts with MINK1. Interacts (GTP-bound form preferentially) with MAP4K4. Interacts with cytoskeletal actin. Ref.12 Ref.14 Ref.15 Ref.16 Ref.17 Ref.20 Ref.21 Ref.24

Subcellular location: Recycling endosome membrane; Lipid-anchor; Cytoplasmic side. Note: May also localize to the Golgi (Ref.11) and the gelatinase-containing granules of neutrophils (Ref.10). Ref.10 Ref.11 Ref.22

Domain: The effector domain mediates the interaction with RUNDC3A

By similarity.

Post-translational modification: Ubiquitinated; undergoes 'Lys-63' monoubiquitination and diubiquitination by NEDD4. Multiple lysine residues are probably modified. Ubiquitination requires TNIK, prevents interaction with effectors and inactivates RAP2A. Ref.24Palmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK

By similarity.

Sequence similarities: Belongs to the small GTPase superfamily. Ras family.