CDKN2C recombinant protein

Recombinant CDKN2C protein

Cat. Num. AAA389433

• Gene Names: CDKN2C; p18; INK4C; p18-INK4C
• Synonyms: CDKN2C; Recombinant CDKN2C protein; Cyclin-dependent kinase 4 inhibitor C;also known as Cyclin-dependent kinase 6 inhibitor;p18-INK4c or p18-INK6; CDKN2C recombinant protein

• Host: E Coli
• Form/Format: Recombinant CDKN2C protein is supplied in 25mM Tris pH7.4, 300mM NaCl, 10% glycerol, 0.5mM TCEP.

• Protein Species: Human
• Tag: His-Tag
• Protein Details: Recombinant CDKN2C protein was expressed in E Colias the full length protein (accession number NP_001253.1) with an N-terminal 6×His-tag.
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant CDKN2C protein 12.5% SDS-PAGE Coomassie staining M.W.: 20.06 kDa Purity: ? 95%)

Related Product Information for CDKN2C recombinant protein

Background: CDKN2C (Cyclin-dependent kinase 4 inhibitor C,also known as Cyclin-dependent kinase 6 inhibitor,p18- INK4c or p18-INK6) is a cyclin kinase inhibitors. Cell cycle regulatory proteins play a critical role in both normal cell growth and tumorigenesis, and the cyclin dependent kinases (CDKs) are the principle regulators of cell cycle progression, and thus are important candidates for therapeutic tumour suppressors. The CDKs, which are responsible for the phosphorylation of the retinoblastoma protein (pRb) and pRb related proteins, are in turn regulated by changes in cyclin levels, phosphorylation and the presence of cyclin kinase inhibitors (CKIs). Two classes of CKIs are present within mammalian cells. One class, the p21 family, consists of p21CIP1/WAF1, p27KIP1 and p57KIP2 which are general inhibitors of the G1S CDKs. Homology between family members is limited to a conserved amino-terminal 60-residue domain responsible for kinase binding and inhibition. The second class of CKIs, the INK4 (Inhibitor of CDK4) family is a group of ankyrin repeat proteins whose members p16INK4a, p15INK4b, p18INK4c and p19INK4d are specific inhibitors of cyclin D1/CDK4 or CDK6 complexes.CDKN2C/p18INK4c as a cyclin kinase inhibitors,Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB.

Product Categories/Family for CDKN2C recombinant protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4502751
• NCBI GeneID: 1031
• NCBI Accession #: NP_001253.1
• NCBI GenBank Nucleotide #: NM_001262.2
• UniProt Accession #: P42773; Q8TB83
• Molecular Weight: 18,127 Da
• NCBI Official Full Name: cyclin-dependent kinase 4 inhibitor C
• NCBI Official Synonym Full Names: cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4)
• NCBI Official Symbol: CDKN2C
• NCBI Official Synonym Symbols: p18; INK4C; p18-INK4C
• NCBI Protein Information: cyclin-dependent kinase 4 inhibitor C; p18-INK6; CDK6 inhibitor p18; cyclin-dependent inhibitor; cyclin-dependent kinase 6 inhibitor p18
• UniProt Protein Name: Cyclin-dependent kinase 4 inhibitor C
• Protein Family: Cyclin-dependent kinase 4 inhibitor
• UniProt Gene Name: CDKN2C
• UniProt Synonym Gene Names: CDKN6
• UniProt Entry Name: CDN2C_HUMAN

NCBI Description

The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to interact with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. Ectopic expression of this gene was shown to suppress the growth of human cells in a manner that appears to correlate with the presence of a wild-type RB1 function. Studies in the knockout mice suggested the roles of this gene in regulating spermatogenesis, as well as in suppressing tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode an identical protein, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

CDKN2C: Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB. Heterodimer of p18 with CDK6. Highest levels found in skeletal muscle. Also found in pancreas and heart. Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.

Protein type: Cell cycle regulation; Protein kinase, regulatory subunit; Inhibitor

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; protein kinase binding

Biological Process: negative regulation of cell proliferation; oligodendrocyte differentiation; negative regulation of phosphorylation; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; negative regulation of cell growth; cell cycle arrest; G1/S transition of mitotic cell cycle