MDH1 recombinant protein

Recombinant MDH1 protein

Cat. Num. AAA389340

• Gene Names: MDH1; MDHA; MOR2; MDH-s; HEL-S-32; MGC:1375
• Synonyms: MDH1; Recombinant MDH1 protein; Malate dehydrogenase cytoplasmic; MDH-s; MDHA; MOR2; MDH1 recombinant protein

• Host: E Coli
• Form/Format: Recombinant MDH1 protein is supplied in 25mM Tris-HCl pH8.0, 300mM NaCl, 10% glycerol and 0.5mM TCEP.

• Protein Species: Human
• Tag: His-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant MDH1 protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 37.5 kDa Purity: >92%)

Related Product Information for MDH1 recombinant protein

Short Description: Full-length human MDH1 protein was expressed in E Coli (accession number NP_005908.1) with a C-terminal 6xHis-tag. The molecular weight of the protein is 37.5 kDa.

Background: MDH1 (Malate Dehydrogenase 1), also called as MDHA or MOR2, catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle, fatty acid synthesis and gluconeogenesis. MDH1 is a cytosolic isozyme, and it is a part of the malate-aspartate shuttle (MAS). MAS is important for intracellular NAD(H) redox homeostasis as it transfers reducing equivalents across the mitochondrial membrane. NAD(H) itself cannot pass the mitochondrial inner membrane, so cytosolic and mitochondrial NAD+/NADH pools are independent from each other, and a proper balance of redox state per compartment is important for cell proliferation and cell growth. A constant supply of cytosolic NAD+ is required to sustain glycolysis via glyceraldehyde-3- phosphate dehydrogenase. Replenishing cytosolic NAD+ occurs via the reversible reduction of oxaloacetate to L-malate by MDH1. Besides, recent studies indicate that MDH1 is overexpressed in several cancers and contributes to PDAC (Pancreatic ductal adenocarcinoma) cell proliferation and metabolism via NAD production to support glycolysis.

Product Categories/Family for MDH1 recombinant protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 5174539
• NCBI GeneID: 4190
• NCBI Accession #: NP_005908.1
• NCBI GenBank Nucleotide #: NM_005917.3
• UniProt Accession #: P40925; Q6I9V0; B2R5V5; B4DUN2; B7Z3I7; F5H098
• Molecular Weight: 38,628 Da
• NCBI Official Full Name: malate dehydrogenase, cytoplasmic isoform 2
• NCBI Official Synonym Full Names: malate dehydrogenase 1, NAD (soluble)
• NCBI Official Symbol: MDH1
• NCBI Official Synonym Symbols: MDHA; MOR2; MDH-s; HEL-S-32; MGC:1375
• NCBI Protein Information: malate dehydrogenase, cytoplasmic; soluble malate dehydrogenase; cytosolic malate dehydrogenase; diiodophenylpyruvate reductase; epididymis secretory protein Li 32
• UniProt Protein Name: Malate dehydrogenase, cytoplasmic
• Protein Family: Malate dehydrogenase
• UniProt Gene Name: MDH1
• UniProt Synonym Gene Names: MDHA
• UniProt Entry Name: MDHC_HUMAN

NCBI Description

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Nov 2010]

Uniprot Description

MDH1: Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Nov 2010]

Protein type: EC 1.1.1.96; Carbohydrate Metabolism - pyruvate; Oxidoreductase; Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.1.1.37; Carbohydrate Metabolism - glyoxylate and dicarboxylate

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: centrosome; extracellular space; mitochondrion; cytoplasm; cytosol

Molecular Function: malic enzyme activity; L-malate dehydrogenase activity; diiodophenylpyruvate reductase activity; NAD binding

Biological Process: malate metabolic process; oxaloacetate metabolic process; NADH metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; glucose metabolic process; cellular carbohydrate metabolic process; pathogenesis; gluconeogenesis