IDH2 recombinant protein

Recombinant IDH2 (R140Q) protein

Cat. Num. AAA388314

• Gene Names: IDH2; IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
• Synonyms: IDH2; Recombinant IDH2 (R140Q) protein; Isocitrate Dehydrogenase (NADP(+)) 2; Mitochondrial; NADP(+)-Specific ICDH; IDH2 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant IDH2 (R140Q) protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant IDH2 (R140Q) protein 10% SDS-PAGE Coomassie staining MW: 52.6 kDa Purity: >95%)

Testing Data

(Recombinant IDH2 (R140Q) protein activity assay 20 uM NADPH and 1 uM a-Ketoglutaric acid were incubated with 500 nM IDH2 (R140Q) protein in a reaction system containing 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10 mM MgCl2 and 0.03% BSA at room temperature. Depletion of NADPH was monitored continuously at absorbance 340 nm for 60 min.)

Related Product Information for IDH2 recombinant protein

Short Description: Recombinant IDH2 (R140Q) protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_002159.2) with a point mutation Arg140Gln and a C-terminal DYKDDDDK-Tag. The molecular weight of the protein is 52.6 kDa. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Background: IDH2 (Isocitrate Dehydrogenase (NADP(+)) 2, Mitochondrial, also known as MNADP-IDH, D2HGA2, IDHM, IDH, IDPM, IDC-M) is a member of isocitrate dehydrogenases, which catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH2 is found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Mutations in IDH1 and IDH2 frequently occur in grade II-III gliomas, secondary glioblastomas and acute myeloid leukemias (AML). To date, mutations in at least four active site arginine residues IDH1 R100, IDH1 R132, IDH2 R140, and IDH2 R172 have been shown to result in the neomorphic production of R(-)-2-hydroxyglutarate (2HG), although these mutants lack the wild-type enzyme's ability to convert isocitrate to a-ketoglutarate (a-KG, 2OG).

Product Categories/Family for IDH2 recombinant protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 28178832
• NCBI GeneID: 3418
• NCBI Accession #: NP_002159.2
• NCBI GenBank Nucleotide #: NM_002168.2
• UniProt Accession #: P48735; Q96GT3; B2R6L6
• Molecular Weight: 452
• NCBI Official Full Name: isocitrate dehydrogenase
• NCBI Official Synonym Full Names: isocitrate dehydrogenase 2 (NADP+), mitochondrial
• NCBI Official Symbol: IDH2
• NCBI Official Synonym Symbols: IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
• NCBI Protein Information: isocitrate dehydrogenase [NADP], mitochondrial; isocitrate dehydrogenase [NADP], mitochondrial; NADP(+)-specific ICDH; oxalosuccinate decarboxylase
• UniProt Protein Name: Isocitrate dehydrogenase [NADP], mitochondrial
• Protein Family: Isocitrate dehydrogenase
• UniProt Gene Name: IDH2
• UniProt Synonym Gene Names: IDH
• UniProt Entry Name: IDHP_HUMAN