SDS-Page
(Recombinant IDH2 protein, SDS-PAGE gel. Recombinant IDH2 protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 51.7 kDa Purity: > 95%)
IDH2 recombinant protein
Recombinant IDH2 protein
Gene Names
IDH2; IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
Synonyms
IDH2; Recombinant IDH2 protein; Isocitrate Dehydrogenase (NADP(+)) 2; Mitochondrial; NADP(+)-Specific ICDH; IDH2 recombinant protein
Host
Baculovirus
Form/Format
Recombinant IDH2 protein is supplied at a concentration of 0.5ug/ul in 25mM HEPES pH7.5, 300mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Activity Assay Conditions: 200uM DL-isocitric acid trisodium and 200uM NADP+ were incubated with IDH2 protein in reaction buffer including 50mM Tris-HCl pH 7.4, 100mM MgCl2 for 10 minutes at room temperature. The generation of product NADPH was monitored by measuring absorbance at 340nm.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant IDH2 protein, SDS-PAGE gel. Recombinant IDH2 protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 51.7 kDa Purity: > 95%)
SDS-Page
(Recombinant IDH2 protein, SDS-PAGE gel. Recombinant IDH2 protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 51.7 kDa Purity: > 95%)
Testing Data
(Recombinant IDH2 protein activity. 200 uM DL-isocitric acid trisodium and 200 uM NADP+ were incubated with IDH2 protein in reaction buffer including 50 mM Tris-HCl pH 7.4, 100 mM MgCl2 for 10 minutes at room temperature. The generation of product NADPH was monitored by measuring absorbance at 340 nm.)
Testing Data
(Recombinant IDH2 protein activity. 200 uM DL-isocitric acid trisodium and 200 uM NADP+ were incubated with IDH2 protein in reaction buffer including 50 mM Tris-HCl pH 7.4, 100 mM MgCl2 for 10 minutes at room temperature. The generation of product NADPH was monitored by measuring absorbance at 340 nm.)
Testing Data
(Recombinant IDH2 protein activity. 200 uM DL-isocitric acid trisodium and 200 uM NADP+ were incubated with IDH2 protein in reaction buffer including 50 mM Tris-HCl pH 7.4, 100 mM MgCl2 for 10 minutes at room temperature. The generation of product NADPH was monitored by measuring absorbance at 340 nm.)
Testing Data
(Recombinant IDH2 protein activity. 200 uM DL-isocitric acid trisodium and 200 uM NADP+ were incubated with IDH2 protein in reaction buffer including 50 mM Tris-HCl pH 7.4, 100 mM MgCl2 for 10 minutes at room temperature. The generation of product NADPH was monitored by measuring absorbance at 340 nm.)
Related Product Information for IDH2 recombinant protein
Short Description: Recombinant IDH2 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_002159.2) with a C-terminal DYKDDDDK-Tag. The molecular weight of the protein is 51.7 kDa. It is suitable for assays in metabolism and pyruvate dehydrogenase complex as well as for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: IDH2 (Isocitrate Dehydrogenase (NADP(+)) 2, Mitochondrial, also known as MNADP-IDH, D2HGA2, IDHM, IDH, IDPM, IDC-M) is a member of isocitrate dehydrogenases, which catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Isocitrate dehydrogenases belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. IDH2 is a NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.
Background: IDH2 (Isocitrate Dehydrogenase (NADP(+)) 2, Mitochondrial, also known as MNADP-IDH, D2HGA2, IDHM, IDH, IDPM, IDC-M) is a member of isocitrate dehydrogenases, which catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Isocitrate dehydrogenases belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. IDH2 is a NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.
Product Categories/Family for IDH2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
452
NCBI Official Full Name
isocitrate dehydrogenase
NCBI Official Synonym Full Names
isocitrate dehydrogenase 2 (NADP+), mitochondrial
NCBI Official Symbol
IDH2
NCBI Official Synonym Symbols
IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
NCBI Protein Information
isocitrate dehydrogenase [NADP], mitochondrial; isocitrate dehydrogenase [NADP], mitochondrial; NADP(+)-specific ICDH; oxalosuccinate decarboxylase
UniProt Protein Name
Isocitrate dehydrogenase [NADP], mitochondrial
Protein Family
UniProt Gene Name
IDH2
UniProt Synonym Gene Names
IDH
UniProt Entry Name
IDHP_HUMAN
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Product Notes
The IDH2 idh2 (Catalog #AAA388312) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "IDH2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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