SDS-Page
(Recombinant JARID1B / KDM5B (2-751) was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 100 kDaPurity: >80%)
JARID1B/KDM5B recombinant protein
Recombinant JARID1B/KDM5B (2-751) protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant JARID1B / KDM5B (2-751) was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 100 kDaPurity: >80%)
SDS-Page
(Recombinant JARID1B / KDM5B (2-751) was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 100 kDaPurity: >80%)
Testing Data
(HTRF assay for JARID1B /KDM5B (2-751) activity 3 uM H3K4me3 (1-21 aa) peptide was incubated with KDM5B (2-751) protein in reaction buffer for 1 hour at room temperature.Anti-H3K4me2-d2 antibody was used to detect reaction product.)
Testing Data
(HTRF assay for JARID1B /KDM5B (2-751) activity 3 uM H3K4me3 (1-21 aa) peptide was incubated with KDM5B (2-751) protein in reaction buffer for 1 hour at room temperature.Anti-H3K4me2-d2 antibody was used to detect reaction product.)
Testing Data
(MALDI-TOF for JARID1B / KDM5B (2-751) activity. 3.3 uM H3K4me3 (1-21 aa) peptide was incubated with 100 nM or 200 nM JARID1B / KDM5B (2-751) protein in reaction buffer for 2 hours at room temperature. MALDI-TOF was used for detection.Catalytic Ability: > 60 turnovers/ enzyme molecule)
Testing Data
(MALDI-TOF for JARID1B / KDM5B (2-751) activity. 3.3 uM H3K4me3 (1-21 aa) peptide was incubated with 100 nM or 200 nM JARID1B / KDM5B (2-751) protein in reaction buffer for 2 hours at room temperature. MALDI-TOF was used for detection.Catalytic Ability: > 60 turnovers/ enzyme molecule)
Background: Lysine (K)-specific demethylase 5B (KDM5B), also known as Jumonji, AT rich interactive domain 1B (JARID1B), is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5B demethylates trimethylated, dimethylated and monomethylated H3K4. It does not demethylate histone H3K9 or H3K27. KDM5B functions as a transcriptional corepressor for FOXG1B and PAX9. KDM5B induces the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, it may act as a tumor suppressor for melanoma.
NCBI and Uniprot Product Information
Uniprot Description
JARID1B: Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma. Interacts with FOXG1B, PAX9, MYC, MYCN and RB1. Interacts with HDAC1, HDAC4, HDAC5 and HDAC7. Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer. Belongs to the JARID1 histone demethylase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cancer Testis Antigen (CTA); Demethylase; EC 1.14.11.-; Oxidoreductase; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: cytoplasm; nucleoplasm; nucleus
Molecular Function: histone demethylase activity; histone demethylase activity (H3-K4 specific); protein binding; transcription corepressor activity; transcription factor activity
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Product Notes
The JARID1B/KDM5B kdm5b (Catalog #AAA388222) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "JARID1B/KDM5B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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