Rabbit JARID1B Polyclonal Antibody | anti-KDM5B antibody

JARID1B

Cat. Num. AAA618499

• Gene Names: KDM5B; CT31; PLU1; PUT1; PLU-1; JARID1B; FLJ10538; FLJ12459; FLJ12491; FLJ16281; FLJ23670; RBBP2H1A
• Reactivity: Human, Monkey
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Synonyms: JARID1B; Polyclonal Antibody; Anti -JARID1B; anti-KDM5B antibody

• Host: Rabbit
• Reactivity: Human, Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of human JARID1B protein. The antibody does not cross-react with other JARID proteins, including JARID1A, JARID1C and JARID1D. Species Crossreactivity: monkey.
• Purity/Purification: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol

• Applicable Applications for anti-KDM5B antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Bot and Immunoprecipitation.; Dilution: Western blot: 1:1000; Immunoprecipitation 1:25
• Immunogen: Synthetic peptide (KLH- coupled) corresponding to the human JARID1B protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from MCF-7, T-47D and COS cells using MBS618499.)

Related Product Information for anti-KDM5B antibody

The methylation state of lysine residues in histone proteins is a major determinant for formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain can catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and alpha-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into 7 separate families (3). The JARID (Jumonji/AT-rich interactive domain-containing protein) family contains four members: JARID1A (also RBP2 and RBBP2), JARID1B (also PLU-1), JARID1C (also SMCX) and JARID1D (also SMCY) (4). In addition to the JmJC domain, these proteins contain JmJN, BRIGHT, C5HC2 zinc-finger, and PHD domains, the latter of which binds to methylated histone H3 (Lys9) (4).

All four JARID proteins demethylate di- and tri-methyl histone H3 Lys4; JARID1B also demethylates mono-methyl histone H3 Lys4 (5-7). JARID1A is a critical RB-interacting protein and is required for Polycomb- Repressive Complex 2 (PRC2)-mediated transcriptional repression during ES cell differentiation (8). A JARID1A-NUP98 gene fusion is associated with myeloid leukemia (9). JARID1B, which interacts with many proteins including c-Myc and HDAC4, may play a role in cell fate decisions by blocking terminal differentiation (10-12). JARID1B is over-expressed in many breast cancers and may act by repressing multiple tumor suppressor genes including BRCA1 and HOXA5 (13,14). JARID1C has been found in a complex with HDAC1, HDAC2, G9a and REST, which binds to and represses REST target genes in non-neuronal cells (7). JARID1C mutations are associated with X-linked mental retardation and epilepsy (15,16). JARID1D is largely uncharacterized.

Product Categories/Family for anti-KDM5B antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 119611841
• NCBI GeneID: 10765
• UniProt Accession #: O94800; Q6UGB8; Q9NSZ7; Q9UFC7; Q9UFD3; Q9UGL1; Q9UIW7
• Molecular Weight: 48,638 Da
• NCBI Official Full Name: Jumonji, AT rich interactive domain 1B (RBP2-like), isoform CRA_d
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 5B
• NCBI Official Symbol: KDM5B
• NCBI Official Synonym Symbols: CT31; PLU1; PUT1; PLU-1; JARID1B; FLJ10538; FLJ12459; FLJ12491; FLJ16281; FLJ23670; RBBP2H1A
• NCBI Protein Information: lysine-specific demethylase 5B; RBP2-H1; OTTHUMP00000038815; OTTHUMP00000038816; OTTHUMP00000197658; cancer/testis antigen 31; histone demethylase JARID1B; putative DNA/chromatin binding motif; jumonji, AT rich interactive domain 1B; jumonji/ARID domain-containing protein 1B; retinoblastoma-binding protein 2 homolog 1; retinoblastoma-binding protein 2, homolog 1A
• UniProt Protein Name: HRIHFB2060 protein
• Protein Family: Lysine-specific demethylase
• UniProt Gene Name: HRIHFB2060
• UniProt Entry Name: O94800_HUMAN

Uniprot Description

JARID1B: Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma. Interacts with FOXG1B, PAX9, MYC, MYCN and RB1. Interacts with HDAC1, HDAC4, HDAC5 and HDAC7. Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer. Belongs to the JARID1 histone demethylase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.11.-; Demethylase; Transcription, coactivator/corepressor; Oxidoreductase; Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; transcription factor activity; transcription corepressor activity

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; rhythmic process; negative regulation of transcription, DNA-dependent