PRDM6 recombinant protein

Recombinant PRDM6 protein

Cat. Num. AAA388205

• Gene Names: PRDM6; PDA3; KMT8C; PRISM
• Purity: The recombinant protein is >75% pure by SDS-PAGE.
• Synonyms: PRDM6; Recombinant PRDM6 protein; PFM3; PRDM6 recombinant protein

• Host: Baculovirus
• Purity/Purification: The recombinant protein is >75% pure by SDS-PAGE.
• Form/Format: Full length recombinant PRDM6 protein was expressed in Sf9 cells and is supplied in 25mM Hepes pH7.5, 300mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant PRDM6 protein gel. Recombinant PRDM6 run on an 8% SDS-PAGE gel and stained with Coomassie blue.)

Related Product Information for PRDM6 recombinant protein

Short Description: Recombinant PRDM6 protein (accession number NP_001129711.1) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK-Tag with a molecular weight of 64.4 kDa. The recombinant protein is >75% pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Background: PRDM6 (PR Domain Containing 6) is a putative histone methyltransferase that acts as a transcriptional repressor of smooth muscle gene expression. PRDM6 can regulate the phenotypic plasticity of smooth muscle cells by suppressing differentiation and maintaining the proliferative potential of vascular SMCs. It also plays a role in endothelial cells by inhibiting endothelial cell proliferation, survival and differentiation.

Product Categories/Family for PRDM6 recombinant protein

Recombinant Proteins; Histone Methyltransferase (HMT); Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 210031233
• NCBI GeneID: 93166
• NCBI Accession #: NP_001129711.1
• NCBI GenBank Nucleotide #: NM_001136239.3
• UniProt Accession #: Q9NQX0; Q9NQW9; B5MCJ4
• Molecular Weight: 46,635 Da
• NCBI Official Full Name: putative histone-lysine N-methyltransferase PRDM6
• NCBI Official Synonym Full Names: PR/SET domain 6
• NCBI Official Symbol: PRDM6
• NCBI Official Synonym Symbols: PDA3; KMT8C; PRISM
• NCBI Protein Information: putative histone-lysine N-methyltransferase PRDM6
• UniProt Protein Name: Putative histone-lysine N-methyltransferase PRDM6
• Protein Family: Putative histone-lysine N-methyltransferase
• UniProt Gene Name: PRDM6
• UniProt Synonym Gene Names: PFM3

NCBI Description

The protein encoded by this gene is a transcriptional repressor and a member of the PRDM family. Family members contain a PR domain and multiple zinc-finger domains. The encoded protein is involved in regulation of vascular smooth muscle cells (VSMC) contractile proteins. Mutations in this gene result in patent ductus arteriosus 3 (PDA3). [provided by RefSeq, Apr 2017]

Uniprot Description

PRDM6: Putative histone methyltransferase that acts as a transcriptional repressor of smooth muscle gene expression. Promotes the transition from differentiated to proliferative smooth muscle by suppressing differentiation and maintaining the proliferative potential of vascular smooth muscle cells. Also plays a role in endothelial cells by inhibiting endothelial cell proliferation, survival and differentiation. It is unclear whether it has histone methyltransferase activity in vivo. According to some authors, it does not act as a histone methyltransferase by itself and represses transcription by recruiting EHMT2/G9a. According to others, it possesses histone methyltransferase activity when associated with other proteins and specifically methylates 'Lys-20' of histone H4 in vitro. 'Lys-20' methylation represents a specific tag for epigenetic transcriptional repression. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Cell development/differentiation; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: nucleus

Molecular Function: histone-lysine N-methyltransferase activity; metal ion binding; nucleic acid binding; protein homodimerization activity

Biological Process: negative regulation of smooth muscle cell differentiation; negative regulation of transcription, DNA-dependent; neurogenesis; transcription, DNA-dependent

Disease: Patent Ductus Arteriosus 3