SDS-Page
(Recombinant PHIP (1302-1434) protein gel. PHIP (1302-1434) protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.)
PHIP recombinant protein
Recombinant PHIP (1302-1434) protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant PHIP (1302-1434) protein gel. PHIP (1302-1434) protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-Page
(Recombinant PHIP (1302-1434) protein gel. PHIP (1302-1434) protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.)
Testing Data
(HTRF assay for Recombinant PHIP (1302-1434) protein activity. 3 uM histone peptide H4K5/8/12/16 (ac4) was incubated with PHIP (1302-1434) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(HTRF assay for Recombinant PHIP (1302-1434) protein activity. 3 uM histone peptide H4K5/8/12/16 (ac4) was incubated with PHIP (1302-1434) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(HTRF assay for Recombinant PHIP (1302-1434) protein activity. 3 uM histone peptide H4K5/8/12/16 (ac4) was incubated with PHIP (1302-1434) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(HTRF assay for Recombinant PHIP (1302-1434) protein activity. 3 uM histone peptide H4K5/8/12/16 (ac4) was incubated with PHIP (1302-1434) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Background: PH-interacting protein (PHIP), also known as WDR11 or BRWD2, is a member of the WD repeat protein family. This family is characterized by regions of approximately 40 amino acids bracketed by gly-his and trp-asp (GH-WD), that potentially facilitate the formation of heterotrimeric or multimeric protein complexes. They also contain bromodomains that recognize acetylated histone lysine residues and function as 'readers' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. PHIP was isolated as a factor that binds to the pleckstrin homology (PH) domain of insulin receptor substrate-1 (IRS-1). PHIP was determined not to be a substrate of the insulin receptor but may instead regulate the insulin and insulin-like growth factor signaling pathways by linking IRS-1 to the insulin receptor. PHIP also stimulates cell proliferation through regulation of cyclin transcription and anti-apoptotic activity through AKT1 phosphorylation and activation. PHIP has also been shown to regulate cell morphology and cytoskeletal organization. In addition, PHIP has been implicated as a positive regulator of beta cell mitogenesis and survival.
NCBI and Uniprot Product Information
Uniprot Description
PHIP: Probable regulator of the insulin and insulin-like growth factor signaling pathways. Stimulates cell proliferation through regulation of cyclin transcription and has an anti- apoptotic activity through AKT1 phosphorylation and activation. Plays a role in the regulation of cell morphology and cytoskeletal organization. Interacts with IRS1 and IRS2. Interacts (via bromo domain) with acetylated lysine residues on histone H1.4, histone H3 and H4 (in vitro)
Chromosomal Location of Human Ortholog: 6q14
Cellular Component: nucleus
Molecular Function: protein binding; insulin receptor binding
Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; regulation of cell shape; regulation of protein amino acid phosphorylation; positive regulation of mitosis; protein import into nucleus; regulation of growth; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; insulin receptor signaling pathway; regulation of cell morphogenesis; cytoskeleton organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation; negative regulation of apoptosis
Similar Products
Product Notes
The PHIP phip (Catalog #AAA388089) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PHIP, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
