Typical Testing Data/Standard Curve (for reference only)
Rat Hemojuvelin ELISA Kit | HFE2 elisa kit
Rat Hemojuvelin ELISA Kit
Gene Names
Hfe2; Rgmc
Reactivity
Rat
Synonyms
Hemojuvelin; Rat Hemojuvelin ELISA Kit; HJV; RGMC; Hemochromatosis type 2 protein; RGM domain family member C; HFE2 elisa kit
Reactivity
Rat
Specificity
The Rat Hemojuvelin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat Hemojuvelin proteins within the range of 62.5 pg/ml - 4000 pg/ml.
Sequence Length
422
Samples
Cell Culture Supernates, Serum, Plasma
Sensitivity
<12 pg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HFE2 elisa kit
Principle of the Assay: The Rat Hemojuvelin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Hemojuvelin in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Rat Hemojuvelin coated on a 96-well plate. Standards and samples are pipetted into the wells and Hemojuvelin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Hemojuvelin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Hemojuvelin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background: Hemojuvelin (HJV/RGMc/HFE2) is a membrane-bound and soluble protein in mammals. It is a member of the repulsive guidance molecule family of proteins. The product of this gene is involved in iron metabolism. It may be a component of the signaling pathway which activates hepcidin or it may act as a modulator of hepcidin expression. It could also represent the cellular receptor for hepcidin. Two uORFs in the 5' UTR negatively regulate the expression and activity of the encoded protein. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Defects in this gene are the cause of hemochromatosis type 2A, also called juvenile hemochromatosis (JH). JH is an early-onset autosomal recessive disorder due to severe iron overload resulting in hypogonadotrophic hypogonadism, hepatic fibrosis or cirrhosis and cardiomyopathy, occurring typically before age of 30.
Background: Hemojuvelin (HJV/RGMc/HFE2) is a membrane-bound and soluble protein in mammals. It is a member of the repulsive guidance molecule family of proteins. The product of this gene is involved in iron metabolism. It may be a component of the signaling pathway which activates hepcidin or it may act as a modulator of hepcidin expression. It could also represent the cellular receptor for hepcidin. Two uORFs in the 5' UTR negatively regulate the expression and activity of the encoded protein. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Defects in this gene are the cause of hemochromatosis type 2A, also called juvenile hemochromatosis (JH). JH is an early-onset autosomal recessive disorder due to severe iron overload resulting in hypogonadotrophic hypogonadism, hepatic fibrosis or cirrhosis and cardiomyopathy, occurring typically before age of 30.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
45,221 Da
NCBI Official Full Name
Hemojuvelin
NCBI Official Synonym Full Names
hemochromatosis type 2 (juvenile)
NCBI Official Symbol
Hfe2
NCBI Official Synonym Symbols
Rgmc
NCBI Protein Information
hemojuvelin
UniProt Protein Name
Hemojuvelin
Protein Family
UniProt Gene Name
Hfe2
UniProt Synonym Gene Names
Rgmc
UniProt Entry Name
RGMC_RAT
Uniprot Description
Acts as a bone morphogenetic protein (BMP) coreceptor. Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis.
Research Articles on HFE2
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Product Notes
The Rat HFE2 hfe2 (Catalog #AAA8248481) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8248481 ELISA Kit recognizes Rat HFE2. It is sometimes possible for the material contained within the vial of "Hemojuvelin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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