SDS-Page
(Recombinant EGFR protein tested by SDS-PAGE gel. 0.5 ug of EGFR protein was analyzed using SDS/PAGE followed by Western blot with either an anti-EGFR or anti-GST antibody.)
EGFR recombinant protein
Recombinant EGFR protein
Purity
Purified by GSH-agarose affinity purification.
Synonyms
EGFR; Recombinant EGFR protein; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; EGFR recombinant protein
Host
Baculovirus
Purity/Purification
Purified by GSH-agarose affinity purification.
Form/Format
10ug of Recombinant EGFR protein in 50mM Tris-HCl, pH8.0; 100mM NaCl, 5mM DTT, 15mM reduced glutathione, 20% glycerol.
Protein Species
Human
Tag
GST-Tag, His-Tag
Notes
Recombinant EGFR is suitable for kinase assays and Western blot. The activity of the protein is ~ 93 pmol/ug min. Recommended kinase reaction conditions: 60mM HEPES-NaOH, pH 7.5, 3mM MgCl2, 3mM MnCl2, 3uM Na-orthovanadate, 1.2mM DTT, ATP (variable), 2.5ug/50ul PEG20.000, Substrate: PolyEY 10ug/50ul, Recombinant EGFR: 40 ng/50ul. Kinase activity may vary depending on the substrate and reaction conditions used.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant EGFR protein tested by SDS-PAGE gel. 0.5 ug of EGFR protein was analyzed using SDS/PAGE followed by Western blot with either an anti-EGFR or anti-GST antibody.)
SDS-Page
(Recombinant EGFR protein tested by SDS-PAGE gel. 0.5 ug of EGFR protein was analyzed using SDS/PAGE followed by Western blot with either an anti-EGFR or anti-GST antibody.)
Related Product Information for EGFR recombinant protein
Short Description: Recombinant human EGFR protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids H672-A1210, accession number NM_005228 and N-terminally fused to GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification. Recombinant EGFR is suitable for kinase assays and Western blot.
Background: EGFR (Epidermal Growth Factor Receptor) are receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin Isoform 2 may act as an antagonist of EGF action.
Background: EGFR (Epidermal Growth Factor Receptor) are receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin Isoform 2 may act as an antagonist of EGF action.
Product Categories/Family for EGFR recombinant protein
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~91.002kDa
Protein Family
Similar Products
Product Notes
The EGFR (Catalog #AAA388024) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "EGFR, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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