Human Sulfotransferase ELISA Kit | CHST2 elisa kit

Human Sulfotransferase ELISA Kit

Cat. Num. AAA3803992

• Gene Names: CHST2; C6ST; GST2; GST-2; Gn6ST-1; HEL-S-75; glcNAc6ST-1
• Reactivity: Human
• Synonyms: Sulfotransferase; Human Sulfotransferase ELISA Kit; CHST2 elisa kit

• Reactivity: Human
• Sequence Length: 530

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 0.625ng/ml-10ng/ml
• Sensitivity: 0.1 ng/ml

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHST2 elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofSULFOTRANSFERASEin the sample, This SULFOTRANSFERASEELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus SULFOTRANSFERASEconcentration. The concentration of SULFOTRANSFERASEin the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27369497
• NCBI GeneID: 9435
• NCBI Accession #: NP_004258
• NCBI GenBank Nucleotide #: NP_004258.2
• UniProt Accession #: Q9Y4C5
• NCBI Official Full Name: carbohydrate sulfotransferase 2
• NCBI Official Synonym Full Names: carbohydrate sulfotransferase 2
• NCBI Official Symbol: CHST2
• NCBI Official Synonym Symbols: C6ST; GST2; GST-2; Gn6ST-1; HEL-S-75; glcNAc6ST-1
• NCBI Protein Information: carbohydrate sulfotransferase 2
• UniProt Protein Name: Carbohydrate sulfotransferase 2
• Protein Family: Sulfotransferase
• UniProt Gene Name: CHST2
• UniProt Synonym Gene Names: GN6ST; GST-2; GlcNAc6ST-1; Gn6ST-1
• UniProt Entry Name: CHST2_HUMAN

NCBI Description

This locus encodes a sulfotransferase protein. The encoded enzyme catalyzes the sulfation of a nonreducing N-acetylglucosamine residue, and may play a role in biosynthesis of 6-sulfosialyl Lewis X antigen. [provided by RefSeq, Aug 2011]

Uniprot Description

CHST2: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Membrane protein, integral; Transferase; EC 2.8.2.-; Glycan Metabolism - keratan sulfate biosynthesis

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: Golgi membrane; intrinsic to Golgi membrane; integral to membrane; trans-Golgi network

Molecular Function: sulfotransferase activity; N-acetylglucosamine 6-O-sulfotransferase activity

Biological Process: keratan sulfate metabolic process; sulfur metabolic process; glycosaminoglycan metabolic process; multicellular organismal development; carbohydrate metabolic process; keratan sulfate biosynthetic process; N-acetylglucosamine metabolic process; pathogenesis; inflammatory response