Human Deiodinase, Iodothyronine, Type I (DIO1) ELISA Kit | DIO1 elisa kit

Human Deiodinase, Iodothyronine, Type I (DIO1) ELISA Kit

Cat. Num. AAA3803827

• Gene Names: Dio1; 5DI; ITDI1; TXDI1
• Reactivity: Human
• Synonyms: Deiodinase; Iodothyronine; Type I (DIO1); Human Deiodinase; Type I (DIO1) ELISA Kit; DIO1 elisa kit

• Reactivity: Human
• Sequence Length: 161

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 0.625ng/ml-10ng/ml
• Sensitivity: 0.1 ng/ml

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DIO1 elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofDIO1in the sample, This DIO1ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus DIO1concentration. The concentration of DIO1in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1023176906
• NCBI GeneID: 25430
• NCBI Accession #: NP_001311139.1
• NCBI GenBank Nucleotide #: NP_001311139.1
• UniProt Accession #: P24389
• NCBI Official Full Name: type I iodothyronine deiodinase isoform 2
• NCBI Official Synonym Full Names: iodothyronine deiodinase 1
• NCBI Official Symbol: Dio1
• NCBI Official Synonym Symbols: 5DI; ITDI1; TXDI1
• NCBI Protein Information: type I iodothyronine deiodinase
• UniProt Protein Name: Type I iodothyronine deiodinase
• Protein Family: Type I iodothyronine deiodinase
• UniProt Gene Name: Dio1
• UniProt Synonym Gene Names: Itdi1; Txdi1

NCBI Description

The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the activation, as well as the inactivation of thyroid hormone by outer and inner ring deiodination, respectively. The activation reaction involves the conversion of the prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4), secreted by the thyroid gland, to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by 5'-deiodination. This protein is expressed predominantly in the liver and kidney and provides most of the circulating T3, which is essential for growth, differentiation and basal metabolism in vertebrates. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2016]

Uniprot Description

Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2 (3,3'-diiodothyronine).