Rabbit anti-Human CTCF Polyclonal Antibody | anti-CTCF antibody

CTCF Antibody - C-terminal region

Cat. Num. AAA3219698

• Gene Names: CTCF; MRD21
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: CTCF; Polyclonal Antibody; CTCF Antibody - C-terminal region; anti-CTCF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Affinity purified
• Form/Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 16% sucrose.
• Sequence: Synthetic peptide located within the following region: PQPVTPAPPPAKKRRGRPPGRTNQPKQNQPTAIIQVEDQNTGAIENIIVE
• Sequence Length: 727

• Applicable Applications for anti-CTCF antibody: Western Blot (WB)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CTCF
• Preparation and Storage: For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Host: RabbitTarget Name: CTCFSample Tissue: Jurkat Whole Cell lysatesAntibody Dilution: 1ug/ml)

Related Product Information for anti-CTCF antibody

This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-CTCF antibody

Polyclonal; Cancer; Transcription Regulation; DNA/RNA/Protein Interactions

NCBI and Uniprot Product Information

• NCBI GI #: 300388142
• NCBI GeneID: 10664
• NCBI Accession #: NP_001177951.1
• NCBI GenBank Nucleotide #: NM_001191022.1
• UniProt Accession #: P49711
• Molecular Weight: 83 kDa
• NCBI Official Full Name: transcriptional repressor CTCF isoform 2
• NCBI Official Synonym Full Names: CCCTC-binding factor
• NCBI Official Symbol: CTCF
• NCBI Official Synonym Symbols: MRD21
• NCBI Protein Information: transcriptional repressor CTCF
• UniProt Protein Name: Transcriptional repressor CTCF
• Protein Family: Transcriptional repressor
• UniProt Gene Name: CTCF
• UniProt Entry Name: CTCF_HUMAN

NCBI Description

This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

CTCF: a widely expressed, sequence-specific DNA-binding transcriptional regulator, insulator, and organizer of higher-order chromatin structure. Acts as a tumor suppressor. Contains 11 C2H2-type zinc fingers. Involved in promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Mediates pairing between X chromosomes and interactions between distant regulatory elements. Binds to promoters of c-myc, PLK, PIM1 and APP. A critical regulator of cell-cycle arrest and death after B cell receptor signaling in immature B cells. CTCF, together with YY1 and Tsix, form a regulated epigenetic switch for X-inactivation.

Protein type: Tumor suppressor; C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 16q21-q22.3

Cellular Component: nucleoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region

Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; chromatin insulator sequence binding; transcription factor activity; transcription corepressor activity

Biological Process: genetic imprinting; transcription, DNA-dependent; maintenance of DNA methylation; positive regulation of transcription, DNA-dependent; regulation of histone methylation; regulation of histone acetylation; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; regulation of gene expression, epigenetic; nucleosome positioning; chromosome segregation; regulation of molecular function, epigenetic; DNA methylation; negative regulation of transcription, DNA-dependent

Disease: Mental Retardation, Autosomal Dominant 21