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Western Blot (WB) (Western blot analysis of RAW264.7 and NIH/3T3 cell lysates using PDIA4 Polyclonal Antibody at dilution of 1:250)

Rabbit PDIA4 Polyclonal Antibody | anti-PDIA4 antibody

PDIA4 Polyclonal Antibody

Gene Names
PDIA4; ERP70; ERP72; ERp-72
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Antigen Affinity Purification
Synonyms
PDIA4; Polyclonal Antibody; PDIA4 Polyclonal Antibody; Calcium binding protein intestinal related; Endoplasmic reticulum resident protein 70; Endoplasmic reticulum resident protein 72; EPR70; EPR72; ER protein 70; ER protein 72; ERP 70; ERp 72; ERp-72; ERp70; ERp72; PDIA 4; Pdia4; PDIR; Protein disulfide isomerase A4; Protein disulfide isomerase associated 4; Protein disulfide isomerase family A member 4; Protein disulfide isomerase related protein (calcium-binding protein; Intestinal-related); Protein disulfide isomerase related protein; Protein disulfide-isomerase A4; Protein ERp 72; Protein ERp72; anti-PDIA4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purification
Form/Format
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Concentration
0.72mg/mL (varies by lot)
Sequence Length
645
Applicable Applications for anti-PDIA4 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:40-1:200
ELISA: 1:5000-1:10000
Immunogen
Fusion protein of human PDIA4
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of RAW264.7 and NIH/3T3 cell lysates using PDIA4 Polyclonal Antibody at dilution of 1:250)

Western Blot (WB) (Western blot analysis of RAW264.7 and NIH/3T3 cell lysates using PDIA4 Polyclonal Antibody at dilution of 1:250)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human tonsil tissue using PDIA4 Polyclonal Antibody at dilution of 1:40(×200))

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human tonsil tissue using PDIA4 Polyclonal Antibody at dilution of 1:40(×200))

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PDIA4 Polyclonal Antibody at dilution of 1:40(×200))

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PDIA4 Polyclonal Antibody at dilution of 1:40(×200))
Related Product Information for anti-PDIA4 antibody
PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC. The full length protein has a signal peptide with 20 amino acid.
Product Categories/Family for anti-PDIA4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
73 kDa
NCBI Official Full Name
Protein disulfide-isomerase A4
NCBI Official Synonym Full Names
protein disulfide isomerase family A member 4
NCBI Official Symbol
PDIA4
NCBI Official Synonym Symbols
ERP70; ERP72; ERp-72
NCBI Protein Information
protein disulfide-isomerase A4
UniProt Protein Name
Protein disulfide-isomerase A4
UniProt Gene Name
PDIA4
UniProt Synonym Gene Names
ERP70; ERP72; ER protein 70; ERp70; ER protein 72; ERp-72; ERp72
UniProt Entry Name
PDIA4_HUMAN

NCBI Description

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. This protein, when bound to cyclophilin B, enhances the rate of immunoglobulin G intermolecular disulfide bonding and antibody assembly. [provided by RefSeq, Dec 2016]

Uniprot Description

PDIA4: Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Belongs to the protein disulfide isomerase family.

Protein type: EC 5.3.4.1; Isomerase; Chaperone; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 7q35

Cellular Component: cell surface; endoplasmic reticulum; endoplasmic reticulum lumen; melanosome

Molecular Function: protein binding; protein disulfide isomerase activity

Biological Process: protein folding; cell redox homeostasis; protein secretion

Research Articles on PDIA4

Similar Products

Product Notes

The PDIA4 pdia4 (Catalog #AAA2560743) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDIA4 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PDIA4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:40-1:200 ELISA: 1:5000-1:10000. Researchers should empirically determine the suitability of the PDIA4 pdia4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDIA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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