Rabbit SOX2 Polyclonal Antibody | anti-SOX2 antibody

SOX2 antibody - N-terminal region

Cat. Num. AAA3200571

• Gene Names: SOX2; ANOP3; MCOPS3
• Reactivity: Cow, Dog, Goat, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep, Zebrafish
• Applications: Immunohistochemistry, Western Blot
• Purity: Affinity Purified
• Synonyms: SOX2; Polyclonal Antibody; SOX2 antibody - N-terminal region; anti-SOX2 antibody

• Host: Rabbit
• Reactivity: Cow, Dog, Goat, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep, Zebrafish
• Clonality: Polyclonal
• Purity/Purification: Affinity Purified
• Form/Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: Batch dependent within range: 100 ul at 0.5 - 1 mg/ml (varies by lot)
• Sequence: Synthetic peptide located within the following region: MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPMNAFMV
• Sequence Length: 317

• Applicable Applications for anti-SOX2 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Homology: Cow: 100%; Dog: 100%; Goat: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 91%
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SOX2
• Protein Size (# AA): 317 amino acids
• Protein Interactions: SUMO1; DMAP1; TBX2; SETD7; FAM21A; FZR1; MMS19; MRPS35; ZNF462; LSM2; CCAR2; MIER1; ZDBF2; CHD8; EP400; TSHZ3; RANBP10; ZNF687; XPO5; INTS2; MTA3; CORO1B; CASC5; MRPS22; KCMF1; MEPCE; TRERF1; INTS9; PARVA; SCYL2; CHD7; ZCCHC8; SOX6; BRIX1; SAMD4B; GID8; C
• Preparation and Storage: For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Human Kidney)

Immunohistochemistry (IHC)

(Human Spleen)

Immunohistochemistry (IHC)

(Researcher:Kim Perry, University of IllinoisApplication:IHCSpecies+tissue/cell type:Xenopus laevis cornea epithelliumPrimary antibody dilution:1:300Secondary antibody:Goat anti-rabbit -rhodamineSecondary antibody dilution:1:300)

Western Blot (WB)

(Host: MouseTarget Name: SOX2Sample Tissue: Mouse LiverAntibody Dilution: 1ug/ml)

Western Blot (WB)

(Host: MouseTarget Name: SOX2Sample Tissue: Mouse PancreasAntibody Dilution: 1ug/ml)

Western Blot (WB)

(Host: RabbitTarget Name: SOX2Sample Tissue: Human 293TAntibody Dilution: 1.0ug/ml)

Western Blot (WB)

(Host: RabbitTarget Name: SOX2Sample Tissue: Mouse LiverAntibody Dilution: 1ug/ml)

Western Blot (WB)

(Sample Type: Lane 1: 20 ug U87 lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:2000Submitted by: Ander Matheu Fernandez, Biodonostia Institute)

Western Blot (WB)

(WB Suggested Anti-SOX2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:500Positive Control: OVCAR-3 cell lysateThere is BioGPS gene expression data showing that SOX2 is expressed in OVCAR3)

Related Product Information for anti-SOX2 antibody

This is a rabbit polyclonal antibody against SOX2. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in this gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation. This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Product Categories/Family for anti-SOX2 antibody

Polyclonal; Transcription Factor; Transcription Regulation; Transcription Regulation; Developmental Biology; Stem Cells; Disease Related; Immunohistochemistry; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 28195386
• NCBI GeneID: 6657
• NCBI Accession #: NP_003097
• NCBI GenBank Nucleotide #: NM_003106
• UniProt Accession #: P48431
• Molecular Weight: 34kDa
• NCBI Official Full Name: transcription factor SOX-2
• NCBI Official Synonym Full Names: SRY-box 2
• NCBI Official Symbol: SOX2
• NCBI Official Synonym Symbols: ANOP3; MCOPS3
• NCBI Protein Information: transcription factor SOX-2
• UniProt Protein Name: Transcription factor SOX-2
• UniProt Gene Name: SOX2
• UniProt Entry Name: SOX2_HUMAN

NCBI Description

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]