Rabbit anti-Human CSH1 Polyclonal Antibody | anti-CSH1 antibody

CSH1, NT (CSH1, Chorionic somatomammotropin hormone, Lactogen, Placental lactogen) (Azide free) (HRP)

Cat. Num. AAA6289279

• Gene Names: CSH1; PL; CSA; CS-1; CSMT; GHB3; hCS-1; hCS-A
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CSH1; Polyclonal Antibody; NT (CSH1; Chorionic somatomammotropin hormone; Lactogen; Placental lactogen) (Azide free) (HRP); Placental lactogen; anti-CSH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CSH1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CSH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-70 amino acids from the N-terminal region of human CSH1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CSH1 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the CSH1 antibody detected the CSH1 protein (arrow).)

Related Product Information for anti-CSH1 antibody

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones and plays an important role in growth control. The gene is located at the growth hormone locus on chromosome 17 along with four other related genes in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed mainly in the placenta and utilizes multiple transcription initiation sites. Expression of the identical mature proteins for chorionic somatomammotropin hormones 1 and 2 is upregulated during development, although the ratio of 1 to 2 increases by term. Mutations in this gene result in placental lactogen deficiency and Silver-Russell syndrome. [provided by RefSeq].

Product Categories/Family for anti-CSH1 antibody

Antibodies; Hormones; Steroids

References

Voorhees, J.L., et al. J. Biol. Chem. 285(26):20022-20030(2010). Mannik, J., et al. J. Clin. Endocrinol. Metab. 95(5):2433-2442(2010). Davila, S., et al. Genes Immun. 11(3):232-238(2010). Varvarigou, A.A., et al. J Perinat Med 37(4):364-369(2009). Chen, Y., et al. Cancer Res. 68(23):9729-9734(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 12408687
• NCBI GeneID: 1442
• NCBI Accession #: NP_072167.1
• NCBI GenBank Nucleotide #: NM_022641.3
• UniProt Accession #: P01243; P0DML3; P0DML2
• NCBI Official Synonym Full Names: chorionic somatomammotropin hormone 1
• NCBI Official Symbol: CSH1
• NCBI Official Synonym Symbols: PL; CSA; CS-1; CSMT; GHB3; hCS-1; hCS-A
• NCBI Protein Information: chorionic somatomammotropin hormone 1
• UniProt Protein Name: Chorionic somatomammotropin hormone
• Protein Family: Chorionic somatomammotropin hormone
• UniProt Gene Name: CSH1
• UniProt Synonym Gene Names: Choriomammotropin; PL
• UniProt Entry Name: CSH_HUMAN

NCBI Description

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones and plays an important role in growth control. The gene is located at the growth hormone locus on chromosome 17 along with four other related genes in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed mainly in the placenta and utilizes multiple transcription initiation sites. Expression of the identical mature proteins for chorionic somatomammotropin hormones 1 and 2 is upregulated during development, although the ratio of 1 to 2 increases by term. Mutations in this gene result in placental lactogen deficiency and Silver-Russell syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

CSH1: Produced only during pregnancy and is involved in stimulating lactation, fetal growth and metabolism. Does not interact with GHR but only activates PRLR through zinc-induced dimerization. Belongs to the somatotropin/prolactin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: extracellular region

Molecular Function: hormone activity; metal ion binding