Rabbit anti-Human SOX2 Polyclonal Antibody | anti-SOX2 antibody

SOX2 (SOX2, Transcription factor SOX-2) (MaxLight 490)

Cat. Num. AAA6349959

• Gene Names: SOX2; ANOP3; MCOPS3
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: SOX2; Polyclonal Antibody; SOX2 (SOX2; Transcription factor SOX-2) (MaxLight 490); Transcription factor SOX-2; anti-SOX2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-SOX2 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: SOX2 antibody is generated from rabbits immunized with a recombinant protein of human SOX2.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SOX2 (arrow) using rabbit polyclonal SOX2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies).)

Flow Cytometry (FC/FACS)

(SOX2 Antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SOX2 antibody

SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.

Product Categories/Family for anti-SOX2 antibody

Antibodies; Transcription Factors

References

Remenyi, A., et al., Genes Dev. 17(16):2048-2059 (2003). Wiebe, M.S., et al., J. Biol. Chem. 278(20):17901-17911 (2003). Fantes, J., et al., Nat. Genet. 33(4):461-463 (2003). Schepers, G.E., et al., Dev. Cell 3(2):167-170 (2002). Kamachi, Y., et al., Trends Genet. 16(4):182-187 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 28195386
• NCBI GeneID: 6657
• NCBI Accession #: NP_003097.1
• NCBI GenBank Nucleotide #: NM_003106.3
• UniProt Accession #: P48431; Q14537
• Molecular Weight: 34kDa
• NCBI Official Full Name: transcription factor SOX-2
• NCBI Official Synonym Full Names: SRY (sex determining region Y)-box 2
• NCBI Official Symbol: SOX2
• NCBI Official Synonym Symbols: ANOP3; MCOPS3
• NCBI Protein Information: transcription factor SOX-2; transcription factor SOX2; SRY-related HMG-box gene 2
• UniProt Protein Name: Transcription factor SOX-2
• UniProt Gene Name: SOX2
• UniProt Entry Name: SOX2_HUMAN