Mouse Neurocan ELISA Kit | NCAN elisa kit

Mouse Neurocan ELISA Kit

Cat. Num. AAA31908

• Gene Names: NCAN; CSPG3
• Reactivity: Mouse
• Synonyms: Neurocan; Mouse Neurocan ELISA Kit; NCAN elisa kit

• Reactivity: Mouse
• Specificity: No significant cross-reactivity or interference between Mouse NCAN and analogues was observed.
• Sequence Length: 1321

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.25 ng/ml - 8 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated NCAN concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse NCAN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NCAN and NCAN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NCAN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for NCAN elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated NCAN concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse NCAN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NCAN and NCAN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NCAN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 118600983
• NCBI GeneID: 1463
• NCBI Accession #: NP_004377.2
• NCBI GenBank Nucleotide #: NM_004386.2
• UniProt Accession #: O14594; Q9UPK6
• Molecular Weight: 143,093 Da
• NCBI Official Full Name: neurocan core protein
• NCBI Official Synonym Full Names: neurocan
• NCBI Official Symbol: NCAN
• NCBI Official Synonym Symbols: CSPG3
• NCBI Protein Information: neurocan core protein; neurocan proteoglycan; chondroitin sulfate proteoglycan 3 (neurocan)
• UniProt Protein Name: Neurocan core protein
• Protein Family: Neurocan core protein
• UniProt Gene Name: NCAN
• UniProt Synonym Gene Names: CSPG3; NEUR
• UniProt Entry Name: NCAN_HUMAN

NCBI Description

Neurocan is a chondroitin sulfate proteoglycan thought to be involved in the modulation of cell adhesion and migration.[supplied by OMIM, Jul 2002]

Uniprot Description

CSPG3: May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid. Belongs to the aggrecan/versican proteoglycan family.

Protein type: Secreted, signal peptide; Cell adhesion; Secreted

Chromosomal Location of Human Ortholog: 19p12

Cellular Component: proteinaceous extracellular matrix; lysosomal lumen; Golgi lumen; extracellular region

Molecular Function: extracellular matrix structural constituent; hyaluronic acid binding; calcium ion binding; carbohydrate binding

Biological Process: axon guidance; extracellular matrix organization and biogenesis; central nervous system development; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; pathogenesis; dermatan sulfate biosynthetic process; chondroitin sulfate metabolic process; chondroitin sulfate catabolic process; carbohydrate metabolic process; regulation of synapse structural plasticity; cell adhesion; skeletal development