Cytomegalovirus (CMV) Antigen | CMV antigen
Cytomegalovirus Grade III
Applications
ELISA, Western Blot
Synonyms
Cytomegalovirus (CMV) Antigen; Cytomegalovirus Grade III; Cytomegalovirus (CMV) Grade III; >60% Viral Protein; CMV antigen
Host
HF Cells
Specificity
Cytomegalovirus (CMV) Grade III, >60% Viral Protein
Form/Format
Liquid
Concentration
Protein Concentration: Acceptable Range: 0.8-1.2mg/ml
Testing Results: 1.0mg/ml (varies by lot)
Testing Results: 1.0mg/ml (varies by lot)
Applicable Applications for CMV antigen
ELISA, Western Blot
Source
Grown in Human Fibroblast Cells
Product manufactures in the United States using only domestic animal materials processed in U.S facilities).
Product manufactures in the United States using only domestic animal materials processed in U.S facilities).
Activity
ELISA Assay: Reference Lot Number: M16961010
Testing Results: Midpoint Titer (test antigen): 840.91
% of Reference: 10.15%
Testing Results: Midpoint Titer (reference antigen): 763.456
Background ELISA Ratio: Aeceptable:< or = 2
Test Result: 1.951
Sensitivity/Specificity Medium Positive Control: Acceptable > or = 3
Test Result: 24.849
Sensitivity/Specificity High Positive Control: Acceptable > or = 8
Test Result: 35.151
Testing Results: Midpoint Titer (test antigen): 840.91
% of Reference: 10.15%
Testing Results: Midpoint Titer (reference antigen): 763.456
Background ELISA Ratio: Aeceptable:< or = 2
Test Result: 1.951
Sensitivity/Specificity Medium Positive Control: Acceptable > or = 3
Test Result: 24.849
Sensitivity/Specificity High Positive Control: Acceptable > or = 8
Test Result: 35.151
Buffer
0.1 M Glycine Buffer, pH 9.3 9.7
Preservative
None
Inactivation
Product inactivated by chemical disruption.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
< or = -65 degree C. Avoid multiple freeze/thaw cycles.
Shipping: Dry Ice.
Shipping: Dry Ice.
Related Product Information for CMV antigen
Viral Strain: AD169
Propagated in HF Cells
Inactivated
Introduction: Cytomegalovirus (CMV) antigen is considered one of the most common viral infections in man. The virus rarely causes disease, but may persist as a latent or chronic infection of the salivary glands, renal tubules, cervix and leukocytes. CMV infections are frequent and occasionally severe in children or adults with congenital or acquired defects of cellular immunity, such as patients with AIDS, cancer and recipients of organ transplants. The transmission of CMV via transfused blood may account for the majority of the CMV infections in organ transplant recipients, neonates and heart surgery patients. Medical conditions resulting from Cytomegalovirus infections include: interstitial pneumonia, mononucleosis (heterophile negative), congenital defects, abortion, mental retardation, blindness and deafness.
We supplies a variety of Cytomegalovirus (CMV) antigens: CMV, CMV-M, CMV-EXT 2 and our new CMV Grade III. All antigens are propagated in human fibroblast cells and infected with the viral strain, AD169. The antigen preparations are partially purified to reduce host cell components prior to inactivation by the extraction process or ultraviolet light yielding high sensitivity and low background antigens useful in a variety of solid phase immunoassays.
The various CMV antigens are designed by the extraction process to perform best in either an IgG or an IgM solid phase immunoassay but several of the antigens can be used for both IgG and IgM depending on the application.
CMV-M antigens are available at a range of concentrations: #7511 (0.3 - 0.4 mg/mL) and #7090 (~1.0mg/mL). The CMV-M antigen process extracts antigens primarily from the nucleus and endoplasmic reticulum of the infected cells to enhance the amount of antigens reacting with the early IgM antibody. In contrast, the entire cell is extracted for the #7504 CMV (0.2 - 0.3 mg/mL) antigen.
The CMV EXT2 process for #7517 is designed to select the CMV glycoprotein antigens of the infected fibroblast cells. This enriches for the antigens found on the surface of the cell that react with IgG antibodies.
The new CMV Grade III antigen #7507 is purified by column chromatography to enrich for pp65 along with other CMV antigens, see the graphic below. The chromatography purified CMV Grade III has a higher specific activity after the removal of impurities and strong reactivity for both IgG and IgM sera.
Propagated in HF Cells
Inactivated
Introduction: Cytomegalovirus (CMV) antigen is considered one of the most common viral infections in man. The virus rarely causes disease, but may persist as a latent or chronic infection of the salivary glands, renal tubules, cervix and leukocytes. CMV infections are frequent and occasionally severe in children or adults with congenital or acquired defects of cellular immunity, such as patients with AIDS, cancer and recipients of organ transplants. The transmission of CMV via transfused blood may account for the majority of the CMV infections in organ transplant recipients, neonates and heart surgery patients. Medical conditions resulting from Cytomegalovirus infections include: interstitial pneumonia, mononucleosis (heterophile negative), congenital defects, abortion, mental retardation, blindness and deafness.
We supplies a variety of Cytomegalovirus (CMV) antigens: CMV, CMV-M, CMV-EXT 2 and our new CMV Grade III. All antigens are propagated in human fibroblast cells and infected with the viral strain, AD169. The antigen preparations are partially purified to reduce host cell components prior to inactivation by the extraction process or ultraviolet light yielding high sensitivity and low background antigens useful in a variety of solid phase immunoassays.
The various CMV antigens are designed by the extraction process to perform best in either an IgG or an IgM solid phase immunoassay but several of the antigens can be used for both IgG and IgM depending on the application.
CMV-M antigens are available at a range of concentrations: #7511 (0.3 - 0.4 mg/mL) and #7090 (~1.0mg/mL). The CMV-M antigen process extracts antigens primarily from the nucleus and endoplasmic reticulum of the infected cells to enhance the amount of antigens reacting with the early IgM antibody. In contrast, the entire cell is extracted for the #7504 CMV (0.2 - 0.3 mg/mL) antigen.
The CMV EXT2 process for #7517 is designed to select the CMV glycoprotein antigens of the infected fibroblast cells. This enriches for the antigens found on the surface of the cell that react with IgG antibodies.
The new CMV Grade III antigen #7507 is purified by column chromatography to enrich for pp65 along with other CMV antigens, see the graphic below. The chromatography purified CMV Grade III has a higher specific activity after the removal of impurities and strong reactivity for both IgG and IgM sera.
Product Categories/Family for CMV antigen
Similar Products
Product Notes
The CMV (Catalog #AAA318657) is an Antigen produced from HF Cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cytomegalovirus (CMV) Antigen can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Researchers should empirically determine the suitability of the CMV for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytomegalovirus (CMV) Antigen, Antigen" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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