MAP-2 (2a + 2b) Microtubule Associated Protein 2 Monoclonal Antibody | anti-MAP2 antibody

MAb to MAP-2 (2a + 2b)

Cat. Num. AAA312163

• Gene Names: MAP2; MAP2A; MAP2B; MAP2C; DKFZp686I2148
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein G chromatography
• Synonyms: MAP-2 (2a + 2b) Microtubule Associated Protein 2; Monoclonal Antibody; MAb to MAP-2 (2a + 2b); Monoclonal Antibody to Microtubule Associated Protein-2 (MAP-2) (2a + 2b); anti-MAP2 antibody

• Host: Host: Mouse; Source: Cell Culture
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: AP20
• Specificity: MAP-2 (2a + 2b) Microtubule Associated Protein 2; Anti-MAP-2 recognizes (Mr 300kDa) MAP-2 protein from bovine brain. Strongly cross-reacts with MAP-2 from human, rat, mouse and chicken brains. Anti-MAP-2 (Clone AP20) reacts with the high molecular weigh
• Purity/Purification: Protein G chromatography
• Form/Format: Purified, Liquid
• Concentration: 100ug/ml (OD280nm) (varies by lot)
• Sequence Length: 1827

• Applicable Applications for anti-MAP2 antibody: Immunohistochemistry - Formalin, Western Blot
• Application Notes: Can be used in protein blotting and immunohistochemistry, frozen tissues only. Anti-MAP-2 may be used in studies of neuron structure in brain tissue. The suggested working dilution for immunohistochemical staining is approximately 1:500. Each laboratory s
• Affinity Constant: Not determined
• Buffer: 0.01M PBS, pH 7.2 containing 1% BSA.
• Preservative: 0.09% Sodium azide
• Important Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Warnings: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
• Preparation and Storage: Short-term (up to 1 month) store at 2 to 8 degree C. Long-term, aliquot and store at -20 degree C. Avoid multiple freeze/thaw cycles.

Product Categories/Family for anti-MAP2 antibody

Monoclonal Antibodies for Neuroscience

References

• Cacares, A. et al (1984) J. Neuroscience 4:394.
• Peng, I. et al (1986) J. Cell Biol. 102:252.
• Lewis, S.A. et al (1989) Nature 342:498.

NCBI and Uniprot Product Information

• NCBI GI #: 215274255
• NCBI GeneID: 4133
• UniProt Accession #: P11137; Q17S04; Q99975; Q99976; Q16296; Q59FX9; Q6NYC5; Q8IUX2
• Molecular Weight: 199,526 Da
• NCBI Official Full Name: Microtubule-associated protein 2
• NCBI Official Synonym Full Names: microtubule-associated protein 2
• NCBI Official Symbol: MAP2
• NCBI Official Synonym Symbols: MAP2A; MAP2B; MAP2C; DKFZp686I2148
• NCBI Protein Information: microtubule-associated protein 2; MAP-2; OTTHUMP00000163915; OTTHUMP00000163916; OTTHUMP00000165185; OTTHUMP00000208143; OTTHUMP00000208144
• UniProt Protein Name: Microtubule-associated protein 2
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAP2
• UniProt Entry Name: MAP2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq]

Uniprot Description

Function: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

Subcellular location: Cytoplasm › cytoskeleton

Probable.

Post-translational modification: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.9 Ref.10 Ref.11 Ref.12

Sequence similarities: Contains 3 Tau/MAP repeats.