Rabbit MBP tag Polyclonal Antibody | anti-MBPtag antibody

MBP tag Polyclonal Antibody

Cat. Num. AAA3015154

• Gene Names: malE; ECK4026; JW3994; malJ
• Reactivity: Escherichia Coli
• Applications: Western Blot
• Purity: affinity purified by Protein A
• Synonyms: MBP tag; Polyclonal Antibody; MBP tag Polyclonal Antibody; MBP-tag; MBP; MMBP; Maltose Binding Protein; Maltose binding protein tag; ECK4026; JW3994; Mal E; MalE; malJ; Maltodextrin binding protein; Maltose ABC transporter periplasmic protein; Maltose binding periplasmic protein; Periplasmic maltose binding protein; MALE_ECOLI; anti-MBPtag antibody

• Host: Rabbit
• Reactivity: Escherichia Coli
• Clonality: Polyclonal
• Specificity: MBP tag Polyclonal Antibody detects over-expressed or recombinant proteins containing the MBP epitope tag.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-MBPtag antibody: Western Blot (WB)
• Application Notes: WB: 1:500-2000
• Immunogen: KLH conjugated synthetic peptide derived from E. coli Maltose Binding Protein:1-100/396
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Testing Data

Testing Data

Related Product Information for anti-MBPtag antibody

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.

NCBI and Uniprot Product Information

• NCBI GI #: 16131860
• NCBI GeneID: 948538
• NCBI Accession #: NP_418458.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P0AEX9; P02928; Q2M6S0
• Molecular Weight: 43,388 Da
• NCBI Official Full Name: maltose transporter subunit
• NCBI Official Symbol: malE
• NCBI Official Synonym Symbols: ECK4026; JW3994; malJ
• NCBI Protein Information: maltose transporter subunit
• UniProt Protein Name: Maltose-binding periplasmic protein
• UniProt Gene Name: malE

NCBI Description

Biofilm microarray result was validated with RT-PCR. [More information is available at EcoGene: EG10554]. In addition to being the periplasmic substrate-binding component of the maltose ABC transporter, MalE with bound substrate is also able to bind to the chemoreceptor Tar to induce chemotaxis toward maltose. [More information is available at EcoCyc: EG10554].

Uniprot Description

maltose-binding protein: Involved in the high-affinity maltose membrane transport system malEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides

Protein type: Transporter

Cellular Component: ATP-binding cassette (ABC) transporter complex; outer membrane-bounded periplasmic space; periplasmic space

Molecular Function: protein binding; transporter activity

Biological Process: carbohydrate transport; maltodextrin transport; maltose transport; response to DNA damage stimulus; transport