Testing Data
(Tissue/cell: Mouse embryos;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37? for 20 min;
Incubation:Anti-KLF2 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugatedused at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,) was used to stain the cell nuclei)
Rabbit KLF2 Polyclonal Antibody | anti-KLF2 antibody
KLF2 Polyclonal Antibody
Gene Names
KLF2; LKLF
Reactivity
Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep
Applications
Immunocytochemistry
Purity
affinity purified by Protein A
Synonyms
KLF2; Polyclonal Antibody; KLF2 Polyclonal Antibody; Kruppel like factor 2; Kruppel like factor; Kruppel like factor LKLF; Kruppel-like factor 2 (lung); Lung krueppel like factor; Lung Kruppel like zinc finger transcription factor; KLF 2; Klf2; KLF2_HUMAN; Krueppel-like factor 2; Lklf; Lung krueppel-like factor; anti-KLF2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep
Clonality
Polyclonal
Specificity
KLF2 Polyclonal Antibody detects endogenous levels of KLF2 protein.
Purity/Purification
affinity purified by Protein A
Form/Format
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Applicable Applications for anti-KLF2 antibody
Immunocytochemistry (ICC)
Application Notes
ICC: 1:100
Immunogen
KLH conjugated synthetic peptide derived from human KLF2:251-355/355
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.
Testing Data
(Tissue/cell: Mouse embryos;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37? for 20 min; Incubation:Anti-KLF2 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugatedused at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,) was used to stain the cell nuclei)
Testing Data
(Tissue/cell: Mouse embryos;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37? for 20 min;
Incubation:Anti-KLF2 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugatedused at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,) was used to stain the cell nuclei)
Related Product Information for anti-KLF2 antibody
Kruppel-like factors (KLFs) are a family of broadly expressed zinc finger transcription factors. KLF2 regulates T-cell trafficking by promoting expression of the lipid-binding receptor S1P1 (S1PR1; MIM 601974) and the selectin CD62L (SELL; MIM 153240) (summary by Weinreich et al., 2009 [PubMed 19592277]).[supplied by OMIM, Feb 2011]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,420 Da
NCBI Official Full Name
Krueppel-like factor 2
NCBI Official Synonym Full Names
Kruppel-like factor 2
NCBI Official Symbol
KLF2
NCBI Official Synonym Symbols
LKLF
NCBI Protein Information
Krueppel-like factor 2
UniProt Protein Name
Krueppel-like factor 2
Protein Family
UniProt Gene Name
KLF2
UniProt Synonym Gene Names
LKLF
NCBI Description
Kruppel-like factors (KLFs) are a family of broadly expressed zinc finger transcription factors. KLF2 regulates T-cell trafficking by promoting expression of the lipid-binding receptor S1P1 (S1PR1; MIM 601974) and the selectin CD62L (SELL; MIM 153240) (summary by Weinreich et al., 2009 [PubMed 19592277]).[supplied by OMIM, Feb 2011]
Uniprot Description
Transcription factor that binds to the CACCC box in the promoter of target genes such as HBB/beta globin or NOV and activates their transcription.
Research Articles on KLF2
Similar Products
Product Notes
The KLF2 klf2 (Catalog #AAA3015150) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KLF2 Polyclonal Antibody reacts with Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's KLF2 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC). ICC: 1:100. Researchers should empirically determine the suitability of the KLF2 klf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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