Rabbit PKC beta Monoclonal Antibody | anti-PKC antibody

PKC beta Rabbit Monoclonal Antibody

Cat. Num. AAA3014157

• Gene Names: PRKCB; PKCB; PRKCB1; PRKCB2; PKCI(2); PKCbeta; PKC-beta
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Protein A affinity purified
• Synonyms: PKC beta; Monoclonal Antibody; PKC beta Rabbit Monoclonal Antibody; Protein kinase C beta type; PKC-B; PKC-beta; PKCB; PRKCB1; PRKCB; anti-PKC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: This antibody detects endogenous levels of PKC beta and does not cross-react with related proteins.
• Purity/Purification: Protein A affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 673

• Applicable Applications for anti-PKC antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-1:2000; IHC: 1:50-1:200; ICC: 1:50-1:200; IF: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: Recombinant antibody.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PKC beta Rabbit mAb at 1:1000 dilutionLane1:Raji whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PKC beta antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining PKC beta 2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PKC beta antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-PKC beta antibody. Counter stained with hematoxylin.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Hela cells with PKC beta antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

Related Product Information for anti-PKC antibody

PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and gamma) and novel (n) PKC isoforms (delta, epsilon, zeta, eta and theta). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and epsilon are independent of Ca2+. On the other hand, nPKC delta and epsilon, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities.

NCBI and Uniprot Product Information

• NCBI GI #: 20127450
• NCBI GeneID: 5579
• NCBI Accession #: NP_002729.2
• NCBI GenBank Nucleotide #: NP_002729.2
• UniProt Accession #: P05771
• Molecular Weight: ~ 82kDa
• NCBI Official Full Name: protein kinase C beta type isoform 2
• NCBI Official Synonym Full Names: protein kinase C beta
• NCBI Official Symbol: PRKCB
• NCBI Official Synonym Symbols: PKCB; PRKCB1; PRKCB2; PKCI(2); PKCbeta; PKC-beta
• NCBI Protein Information: protein kinase C beta type
• UniProt Protein Name: Protein kinase C beta type
• UniProt Gene Name: PRKCB
• UniProt Synonym Gene Names: PKCB; PRKCB1; PKC-B; PKC-beta
• UniProt Entry Name: KPCB_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCB: an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic receptors. Activates the NF-kappaB signaling pathway in B cells after antigen-receptor signaling. Two splice-variant isoforms have been described.

Protein type: EC 2.7.11.13; Motility/polarity/chemotaxis; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, AGC; AGC group; PKC family; Alpha subfamily

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: nucleoplasm; membrane; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; androgen receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; protein kinase C binding; histone binding; zinc ion binding; calcium channel regulator activity; chromatin binding; ATP binding

Biological Process: platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; B cell activation; apoptosis; positive regulation of B cell receptor signaling pathway; mitotic nuclear envelope disassembly; negative regulation of insulin receptor signaling pathway; signal transduction; protein amino acid phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; activation of NF-kappaB transcription factor; regulation of transcription from RNA polymerase II promoter; cellular calcium ion homeostasis; synaptic transmission; positive regulation of angiogenesis; B cell receptor signaling pathway; lipoprotein transport; calcium ion transport; response to hypoxia; mitotic cell cycle; blood coagulation; vascular endothelial growth factor receptor signaling pathway