Rabbit anti-Human Cdk8 Polyclonal Antibody | anti-Cdk8 antibody

Cdk8, ID (Cdk8, Cyclin-dependent kinase 8, Cell division protein kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8) (FITC)

Cat. Num. AAA6284504

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Cdk8; Polyclonal Antibody; ID (Cdk8; Cyclin-dependent kinase 8; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8) (FITC); Mediator of RNA polymerase II transcription subunit CDK8; anti-Cdk8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-Cdk8 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Cdk8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-272 amino acids from the Central region of Mouse Cdk8.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Cdk8 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Cdk8 antibody detected the Cdk8 protein (arrow).)

Related Product Information for anti-Cdk8 antibody

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation (By similarity).

Product Categories/Family for anti-Cdk8 antibody

Antibodies; Protein Kinases

References

Alarcon, C., et al. Cell 139(4):757-769(2009). Westerling, T., et al. Mol. Cell. Biol. 27(17):6177-6182(2007). Jang, M.K., et al. Mol. Cell 19(4):523-534(2005). Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004). Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(24):14109-14114(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 31652272
• NCBI GeneID: 264064
• NCBI Accession #: NP_705827.2
• NCBI GenBank Nucleotide #: NM_153599.3
• UniProt Accession #: Q8R3L8
• Molecular Weight: 52,611 Da
• NCBI Official Full Name: cyclin-dependent kinase 8
• NCBI Official Synonym Full Names: cyclin-dependent kinase 8
• NCBI Official Symbol: Cdk8
• NCBI Protein Information: cyclin-dependent kinase 8
• UniProt Protein Name: Cyclin-dependent kinase 8
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: Cdk8

Uniprot Description

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation ().