Western Blot (WB)
(Western Blot (WB) analysis of Nrf3 (D530) pAb at 1:500 dilutionLane1:CT26 whole cell lysate (40ug) Lane2:The Uterus tissue lysate of Rat (40ug) Lane3:A375 whole cell lysate (40ug) Lane4:PC3 whole cell lysate (40ug))
Rabbit Nrf3 Polyclonal Antibody | anti-Nrf3 antibody
Nrf3 (D530) Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Nrf3; Polyclonal Antibody; Nrf3 (D530) Polyclonal Antibody; Nuclear factor erythroid 2-related factor 3; NF-E2-related factor 3; NFE2-related factor 3; Nuclear factor; erythroid derived 2; like 3; NFE2L3; NRF3; anti-Nrf3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Nrf3 (D530) polyclonal antibody detects endogenous levels of Nrf3 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
694
Applicable Applications for anti-Nrf3 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic peptide, corresponding to amino acids 500-520 of Human Nrf3.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of Nrf3 (D530) pAb at 1:500 dilutionLane1:CT26 whole cell lysate (40ug) Lane2:The Uterus tissue lysate of Rat (40ug) Lane3:A375 whole cell lysate (40ug) Lane4:PC3 whole cell lysate (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of Nrf3 (D530) pAb at 1:500 dilutionLane1:CT26 whole cell lysate (40ug) Lane2:The Uterus tissue lysate of Rat (40ug) Lane3:A375 whole cell lysate (40ug) Lane4:PC3 whole cell lysate (40ug))
Related Product Information for anti-Nrf3 antibody
Nrf3 (nuclear factor (erythroid-derived 2)-like 3, NFE2L3, NF-E2-related factor 3, NRF3) is a transcription factor that influences placental gene expression and development. Nrf3 contains a Cap'n'collar (CNC)-type basic leucine zipper (bZIP) domain, and belongs to the CNC gene family. CNC genes play important roles in development, differentiation, oncogenesis, and stress signaling. Nrf3 protein is detectable in primary placental cytotrophoblasts. The human choriocarcinoma cell lines BeWo and JAR from trophoblastic tumors of the placenta express Nrf3 transcripts. Nrf3/MAFG heterodimers recognize nuclear factor-erythroid 2/Maf recognition element-type DNA-binding motifs. Keratinocyte growth factor (KGF)-regulates Nrf3-dependent gene expression during cutaneous wound repair.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 76kDa
NCBI Official Full Name
nuclear factor erythroid 2-related factor 3
UniProt Protein Name
Nuclear factor erythroid 2-related factor 3
UniProt Gene Name
NFE2L3
UniProt Synonym Gene Names
NRF3; NF-E2-related factor 3
UniProt Entry Name
NF2L3_HUMAN
Uniprot Description
NFE2L3: Activates erythroid-specific, globin gene expression. Belongs to the bZIP family. CNC subfamily.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 7p15.2
Cellular Component: nucleus
Molecular Function: transcription coactivator activity; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter
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Product Notes
The Nrf3 nfe2l3 (Catalog #AAA3013900) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nrf3 (D530) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Nrf3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the Nrf3 nfe2l3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nrf3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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