Rabbit Na+/K+-ATPase Polyclonal Antibody | anti-ATP1A1 antibody

Na+/K+-ATPase Polyclonal Antibody

Cat. Num. AAA3013093

• Gene Names: ATP1A1; CMT2DD; HOMGSMR2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: Na+/K+-ATPase; Polyclonal Antibody; Na+/K+-ATPase Polyclonal Antibody; Na(+)/K(+) ATPase alpha-1 subunit; ATP1A1; ATP1B1; ATP1B; Na(+)/K(+) ATPase alpha-4 subunit; ATP1A4; ATP1AL2; ATPase Na+/K+ transporting alpha; adenosinetriphosphatase; AT1A1; ATPase Na+/K+ transporting alpha 1 polypeptide; ATPase Na+/K+ transporting alpha 4 polypeptide; ATPase Na+/K+ transporting beta 1 polypeptide; ATPase; Na+/K+ transporting; alpha polypeptide-like 2; beta 1 polypeptide; Beta 1-subunit of Na(+); K(+)-ATPase; Na+; K+ ATPase alpha subunit; Na+/K+ ATPase 1; Na+/K+ ATPase 4; Na+/K+ ATPase; alpha-D polypeptide; Na; K-ATPase beta-1 polypeptide; K-ATPase; alpha-A catalytic polypeptide; K-ATPase catalytic subunit alpha-A protein; K-ATPase subunit alpha-C; polypeptide-like 2; Sodium pump 1; sodium pump 4; Sodium pump subunit alpha-1; sodium pump subunit alpha-4; sodium pump subunit beta-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-4; sodium-potassium ATPase subunit beta 1 (non-catalytic); sodium-potassium ATPase; alpha 4 polypeptide; sodium-potassium-ATPase; alpha 1 polypeptide; Sodium/potassium transporting ATPase alpha 1 chain; Sodium/potassium transporting ATPase subunit beta 1; sodium/potassium-dependent ATPase beta-1 subunit; sodium/potassium-transporting ATPase alpha-4 chain; sodium/potassium-transporting ATPase beta-1 chain; Sodium/potassium-transporting ATPase subunit alpha-1; sodium/potassium-transporting ATPase subunit alpha-4; anti-ATP1A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Na+/K+-ATPase polyclonal antibody detects endogenous levels of Na+/K+-ATPase protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1023

• Applicable Applications for anti-ATP1A1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1,000-1:5,000; ICC: 1:50-1:200; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Sodium Potassium ATPase on A549 cell lysates using anti-Sodium Potassium ATPase antibody at 1/1,000 dilution.)

Immunocytochemistry (ICC)

(ICC staining Sodium Potassium ATPase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-ATP1A1 antibody

The ubiquitously expressed sodium/potassium-ATPase (Na⁺/K⁺-ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na⁺ ions and two K⁺ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na⁺/K⁺-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, alpha, beta and gamma, comprise the Na⁺/K⁺-ATPase oligomer. The alpha subunit contains the binding sites for ATP and the cations; the glycosylated beta subunit ensures correct folding and membrane insertion of the alpha subunits. The small gamma subunit co-localizes with the alpha subunit in nephron segments, where it increases the affinity of Na⁺/K⁺-ATPase for ATP. The beta subunit, but not the gamma subunit, is essential for normal activity of Na⁺/K⁺-ATPase.

NCBI and Uniprot Product Information

• NCBI GI #: 21361181
• NCBI GeneID: 476
• NCBI Accession #: NP_000692.2
• NCBI GenBank Nucleotide #: NP_000692.2
• UniProt Accession #: P05023; P05026; Q13733; P14094; Q8VDN2; Q9WV27; P06685 P07340 Q64541
• Molecular Weight: 100kDa
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit alpha-1 isoform a
• NCBI Official Synonym Full Names: ATPase Na+/K+ transporting subunit alpha 1
• NCBI Official Symbol: ATP1A1
• NCBI Official Synonym Symbols: CMT2DD; HOMGSMR2
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit alpha-1
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit alpha-1
• Protein Family: Sodium/potassium-transporting ATPase
• UniProt Gene Name: ATP1A1
• UniProt Synonym Gene Names: Na(+)/K(+) ATPase alpha-1 subunit
• UniProt Entry Name: AT1A1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

ATP1A1: the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na and K ions across the plasma membrane. This action creates the electrochemical gradient of Na and K, providing the energy for active transport of various nutrients. Two splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Transporter, ion channel; EC 3.6.3.9; Membrane protein, multi-pass; Hydrolase; Transporter

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: Golgi apparatus; protein complex; basolateral plasma membrane; endoplasmic reticulum; T-tubule; integral to membrane; caveola; membrane; apical plasma membrane; plasma membrane; melanosome; sodium:potassium-exchanging ATPase complex; sarcolemma; endosome; vesicle

Molecular Function: potassium ion binding; protein domain specific binding; protein binding; sodium ion binding; chaperone binding; phosphoric monoester hydrolase activity; sodium:potassium-exchanging ATPase activity; phosphoinositide 3-kinase binding; ankyrin binding; ADP binding; protein kinase binding; ATP binding

Biological Process: response to drug; cellular sodium ion homeostasis; negative regulation of heart contraction; membrane hyperpolarization; positive regulation of striated muscle contraction; regulation of sodium ion transport; cellular potassium ion homeostasis; negative regulation of glucocorticoid biosynthetic process; dephosphorylation; potassium ion import; regulation of blood pressure; positive regulation of heart contraction; transmembrane transport; regulation of the force of heart contraction; cardiac muscle contraction