Western Blot (WB)
(Western Blot analysis of HE4 on different lysates using anti-HE4 antibody at 1/1,000 dilution. Positive control: Lane 1: SW480 Lane 2: SKOV-3)
Rabbit anti-Human, Mouse HE4 Polyclonal Antibody | anti-HE4 antibody
HE4 Polyclonal Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
HE4; Polyclonal Antibody; HE4 Polyclonal Antibody; WFDC2; dJ461P17.6; EDDM4; epididymal protein 4; Epididymal secretory protein E4; Epididymis specific whey acidic protein type four disulfide core; HE 4; Major epididymis specific protein E4; Major epididymis-specific protein E4; MGC57529; Putative protease inhibitor WAP5; WAP 5; WAP domain containing protein HE4; WAP domain containing protein HE4 V4; WAP four disulfide core domain 2; WAP four disulfide core domain protein 2; WAP four-disulfide core domain protein 2; WAP5; WFDC 2; anti-HE4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
HE4 polyclonal antibody detects endogenous levels of HE4 protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
124
Applicable Applications for anti-HE4 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
ICC: 1:50-1:200
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of HE4 on different lysates using anti-HE4 antibody at 1/1,000 dilution. Positive control: Lane 1: SW480 Lane 2: SKOV-3)
Western Blot (WB)
(Western Blot analysis of HE4 on different lysates using anti-HE4 antibody at 1/1,000 dilution. Positive control: Lane 1: SW480 Lane 2: SKOV-3)
Immunocytochemistry (ICC)
(ICC staining HE4 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining HE4 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-HE4 antibody
HE4 (whey acidic protein (WAP)-type four-disulfide core-2, WFDC2) is a small secretory protein that may influence sperm maturation. HE4 gene expression is high in pulmonary epithelial cells and in some ovarian cancers. HE4 protein has a WAP motif that contains eight cysteines forming four disulfide bonds at the core of the protein. The WAP motif functions as a protease inhibitor in many of the family members that contain them.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23kDa
NCBI Official Full Name
WAP four-disulfide core domain protein 2
UniProt Protein Name
WAP four-disulfide core domain protein 2
UniProt Gene Name
WFDC2
UniProt Synonym Gene Names
HE4; WAP5
UniProt Entry Name
WFDC2_HUMAN
Uniprot Description
WFDC2: a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 20q13.12
Cellular Component: extracellular space
Molecular Function: serine-type endopeptidase inhibitor activity; aspartic-type endopeptidase inhibitor activity; endopeptidase inhibitor activity; cysteine protease inhibitor activity
Biological Process: spermatogenesis; proteolysis
Similar Products
Product Notes
The HE4 wfdc2 (Catalog #AAA3012810) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HE4 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HE4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1,000-1:2,000 ICC: 1:50-1:200 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the HE4 wfdc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HE4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
