Human HE4 ELISA Kit | WFDC2 elisa kit

Human HE4 PicoKine ELISA Kit

Cat. Num. AAA178534

• Gene Names: WFDC2; HE4; WAP5; EDDM4; dJ461P17.6
• Reactivity: Human
• Synonyms: HE4; Human HE4 PicoKine ELISA Kit; EDDM4; epididymal protein 4; HE 4; WAP 5; WAP5; WFDC 2; WFDC2; Q14508; WAP four-disulfide core domain protein 2; WAP four-disulfide core domain 2; WFDC2 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human WFDC2
• Sequence Length: 124

• Samples: Cell culture supernatants, serum, plasma (heparin, edta), saliva, urine and human milk
• Detection Range: 156 pg/ml - 10,000 pg/ml
• Sensitivity: <15 pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WFDC2 elisa kit

Principle of the Assay: The Picokine™ Human WFDC2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human WFDC2 with a 96-well strip plate that is pre-coated with antibody specific for WFDC2. The detection antibody is a biotinylated antibody specific for WFDC2. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from sheep. The kit contains recombinant Human WFDC2 with immunogen: Expression system for standard: E.coli; Immunogen sequence: E31-F124. The kit is analytically validated with ready-to-use reagents. To measure Human WFDC2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human WFDC2 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human WFDC2 in the sample.

Background/Introduction: WAP four-disulfide core domain protein 2, also known as Human Epididymis Protein 4 (HE4), is a protein that in humans is encoded by the WFDC2 gene. This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.

NCBI and Uniprot Product Information

• NCBI GI #: 56699495
• NCBI GeneID: 10406
• NCBI Accession #: NP_006094.3
• NCBI GenBank Nucleotide #: NM_006103.3
• UniProt Accession #: Q14508; Q6IB27; Q8WXV9; Q8WXW0; Q8WXW1; Q8WXW2; Q96KJ1; A2A2A5; A2A2A6; A6PVD5
• Molecular Weight: 11,043 Da
• NCBI Official Full Name: WAP four-disulfide core domain protein 2
• NCBI Official Synonym Full Names: WAP four-disulfide core domain 2
• NCBI Official Symbol: WFDC2
• NCBI Official Synonym Symbols: HE4; WAP5; EDDM4; dJ461P17.6
• NCBI Protein Information: WAP four-disulfide core domain protein 2
• UniProt Protein Name: WAP four-disulfide core domain protein 2
• Protein Family: WAP four-disulfide core domain protein
• UniProt Gene Name: WFDC2
• UniProt Synonym Gene Names: HE4; WAP5
• UniProt Entry Name: WFDC2_HUMAN

NCBI Description

This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]

Uniprot Description

WFDC2: a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: extracellular space

Molecular Function: aspartic-type endopeptidase inhibitor activity; endopeptidase inhibitor activity; serine-type endopeptidase inhibitor activity

Biological Process: proteolysis; spermatogenesis