Western Blot (WB)
(Western Blot analysis of Cytokeratin 1 on different lysates using anti-Cytokeratin 1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: Hela)
Rabbit Cytokeratin 1 Polyclonal Antibody | anti-KRT1 antibody
Cytokeratin 1 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
Cytokeratin 1; Polyclonal Antibody; Cytokeratin 1 Polyclonal Antibody; CK-1; K1; KRT1; 67 kDa cytokeratin; CK1; Cytokeratin-1; Cytokeratin1; EHK; EHK1; Epidermolytic hyperkeratosis 1; EPPK; Hair alpha protein; K2C1; Keratin; Keratin type II cytoskeletal 1; Keratin-1; Keratin1; KRT 1; Krt1; KRT1A; NEPPK; type II cytoskeletal 1; Type II keratin Kb1; Type-II keratin Kb1; anti-KRT1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Cytokeratin 1 polyclonal antibody detects endogenous levels of Cytokeratin 1 protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
644
Applicable Applications for anti-KRT1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1,000
IHC: 1:100-1:500
IHC: 1:100-1:500
Immunogen
recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of Cytokeratin 1 on different lysates using anti-Cytokeratin 1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: Hela)
Western Blot (WB)
(Western Blot analysis of Cytokeratin 1 on different lysates using anti-Cytokeratin 1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: Hela)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 1 antibody. Counter stained with hematoxylin.)
Related Product Information for anti-KRT1 antibody
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins constitute up to 85% of a mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells. The alpha-helical coiled-coil dimers associate laterally end-to-end to form 10-nm diameter filaments. Cytokeratins are useful markers of tissue differentiation, and they aid in the characterization of malignant tumors. Cytokeratin 1 is highly expressed in several malignancies including epithelioid hemangioendohtheliomas, angiosarcomas, schwannomas, epithelioid sarcomas and synodal sarcomas. The gene encoding human Cytokeratin 1 maps to chromosome 12q13.13. Mutations in the gene encoding human Cytokeratin 1 lead to abnormal filament associations and epidermolytic hyperkeratosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72kDa
NCBI Official Full Name
keratin, type II cytoskeletal 1
UniProt Protein Name
Keratin, type II cytoskeletal 1
Protein Family
UniProt Gene Name
KRT1
UniProt Synonym Gene Names
KRTA; CK-1; K1
Uniprot Description
May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
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Product Notes
The Cytokeratin 1 krt1 (Catalog #AAA3012555) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cytokeratin 1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1,000 IHC: 1:100-1:500. Researchers should empirically determine the suitability of the Cytokeratin 1 krt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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