Rabbit anti-Human, Mouse L3MBTL3 Polyclonal Antibody | anti-L3MBTL3 antibody

L3MBTL3 Polyclonal Antibody

Cat. Num. AAA3012033

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: L3MBTL3; Polyclonal Antibody; L3MBTL3 Polyclonal Antibody; Lethal(3)malignant brain tumor-like protein 3; H-l(3)mbt-like protein 3; L(3)mbt-like protein 3; MBT-1; KIAA1798; MBT1; anti-L3MBTL3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: L3MBTL3 polyclonal antibody detects endogenous levels of L3MBTL3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 780

• Applicable Applications for anti-L3MBTL3 antibody: Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:100; IP: 1:50-1:200
• Immunogen: Recombinant full length Human L3MBTL3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using L3MBTL3 antibody at 1:1000 dilution.)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF7 cell using L3MBTL3 antibody. Blue: DAPI for nuclear staining.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug L3MBTL3 antibody. Western Blot was performed from the immunoprecipitate using L3MBTL3 antibody at a dilition of 1:1000.)

Related Product Information for anti-L3MBTL3 antibody

L3MBTL3 (lethal(3)malignant brain tumor-like protein 3), also known as MBT-1 or KIAA1798, is a 780 amino acid protein containing three MBT repeats and one SAM (sterile alpha motif) domain. Localized to the nucleus, L3MBTL3 interacts with RING1B, another PcG protein that may be involved in the specification of anterior-posterior axis and cell proliferation in early development. L3MBTL3 exists as two isoforms produced by alternative splicing events.

NCBI and Uniprot Product Information

• NCBI GI #: 62900619
• UniProt Accession #: Q96JM7
• Molecular Weight: ~ 110kDa
• NCBI Official Full Name: Lethal(3)malignant brain tumor-like protein 3
• UniProt Protein Name: Lethal(3)malignant brain tumor-like protein 3
• Protein Family: Lethal(3)malignant brain tumor-like protein
• UniProt Gene Name: L3MBTL3
• UniProt Synonym Gene Names: KIAA1798; MBT1; H-l(3)mbt-like protein 3; L(3)mbt-like protein 3
• UniProt Entry Name: LMBL3_HUMAN

Uniprot Description

L3MBTL3: a Polycomb group (PcG) protein that regulates the maturation of myeloid progenitor cells. May influence myelopoiesis by transiently enhancing p57(KIP2) expression levels. PcG proteins participate in DNA-binding complexes with gene-repressing activity, many of which have been highlighted for their involvement in hematopoiesis. A member of the malignant brain tumor (MBT) family of chromatin-interacting transcriptional repressors. Contains a SAM domain, a common protein modules found in eukaryotic proteomes. Many SAM domains have been shown to form helical polymer structures. May create large protein complexes in the cell. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: nucleus

Biological Process: erythrocyte maturation; macrophage differentiation; regulation of transcription, DNA-dependent; granulocyte differentiation; transcription, DNA-dependent; chromatin modification