Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using GTF2H3 antibody at 1:1000 dilution.)
Rabbit GTF2H3 Polyclonal Antibody | anti-GTF2H3 antibody
GTF2H3 Polyclonal Antibody
IF: 1:50-1:100
IP: 1:50-1:200
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using GTF2H3 antibody at 1:1000 dilution.)
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using GTF2H3 antibody at 1:1000 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cell using C1D antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cell using C1D antibody. Blue: DAPI for nuclear staining.)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of 200ug extracts of HT-29 cells using 1ug GTF2H3 antibody (A7188). Western Blot was performed from the immunoprecipitate using GTF2H3 antibody at a dilition of 1:1000.)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of 200ug extracts of HT-29 cells using 1ug GTF2H3 antibody (A7188). Western Blot was performed from the immunoprecipitate using GTF2H3 antibody at a dilition of 1:1000.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the TFB4 family. The encoded protein is a subunit of the core-TFIIH basal transcription factor and localizes to the nucleus. The encoded protein is involved in RNA transcription by RNA polymerase II and nucleotide excision repair and associates with the Cdk-activating kinase complex. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 14. [provided by RefSeq, Dec 2012]
Uniprot Description
GTF2H3: Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Anchors XPB. Belongs to the TFB4 family.
Protein type: Transcription factor; DNA repair, damage
Chromosomal Location of Human Ortholog: 12q24.31
Cellular Component: nucleoplasm; holo TFIIH complex
Molecular Function: RNA polymerase subunit kinase activity; DNA-dependent ATPase activity; translation factor activity, nucleic acid binding; metal ion binding; damaged DNA binding; protein N-terminus binding; transcription factor activity; protein kinase activity
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; translation; viral reproduction; positive regulation of viral transcription; transcription from RNA polymerase I promoter; RNA elongation from RNA polymerase I promoter; DNA repair; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; protein amino acid phosphorylation; mRNA capping; regulation of transcription, DNA-dependent; negative regulation of gene expression, epigenetic; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; nucleotide-excision repair, DNA damage removal; gene expression; transcription initiation from RNA polymerase I promoter
Research Articles on GTF2H3
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Product Notes
The GTF2H3 gtf2h3 (Catalog #AAA3011990) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GTF2H3 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GTF2H3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 IF: 1:50-1:100 IP: 1:50-1:200. Researchers should empirically determine the suitability of the GTF2H3 gtf2h3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GTF2H3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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