Rabbit PGM1 Polyclonal Antibody | anti-PGM1 antibody

PGM1 Polyclonal Antibody

Cat. Num. AAA3011493

• Gene Names: PGM1; CDG1T; GSD14
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PGM1; Polyclonal Antibody; PGM1 Polyclonal Antibody; Phosphoglucomutase-1; PGM 1; Glucose phosphomutase 1; anti-PGM1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PGM1 polyclonal antibody detects endogenous levels of PGM1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 580

• Applicable Applications for anti-PGM1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant full length Human PGM1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PGM1 polyclonal antibody)

Related Product Information for anti-PGM1 antibody

Phosphoglucomutase, which belongs to the phophohexose mutase family, plays a role in glycogen catabolism (glycogenolysis) as well as in the process of glycogen synthesis (glycogenesis). During glycogenolysis, PGM converts glucose-1-phosphate to glucose-6-phosphate, thus promoting glycolysis and the pentose phosphate pathway. During glycogenesis, PGM functions in the opposite manner, converting glucose-6-phosphate into glucose-1-phosphate, to facilitate glycogen synthesis. PGM has three structural loci: PGM1, PGM2 and PGM3. These three genetic forms of PGM differ in amino acid sequences but catalyze the same reactions, therefore indicating that they are isozymes. PGM1, a 562 amino acid protein, is highly polymorphic; three mutations and four intragenic recombination events between the three mutation sites generate eight protein variants. All phosphoglucomutases act as monomers and bind one magnesium ion per subunit.

NCBI and Uniprot Product Information

• NCBI GI #: 290463102
• NCBI GeneID: 5236
• NCBI Accession #: NP_001166289.1
• NCBI GenBank Nucleotide #: NP_001166289.1
• UniProt Accession #: P36871
• Molecular Weight: ~ 61kDa
• NCBI Official Full Name: phosphoglucomutase-1 isoform 2
• NCBI Official Synonym Full Names: phosphoglucomutase 1
• NCBI Official Symbol: PGM1
• NCBI Official Synonym Symbols: CDG1T; GSD14
• NCBI Protein Information: phosphoglucomutase-1
• UniProt Protein Name: Phosphoglucomutase-1
• Protein Family: Phosphoglucomutase
• UniProt Gene Name: PGM1
• UniProt Synonym Gene Names: PGM 1
• UniProt Entry Name: PGM1_HUMAN

NCBI Description

The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Mar 2010]

Uniprot Description

This enzyme participates in both the breakdown and synthesis of glucose.