Rabbit ZEB2 Polyclonal Antibody | anti-ZEB2 antibody

Rabbit Polyclonal to Human ZEB2

Cat. Num. AAA243094

• Gene Names: ZEB2; SIP1; SIP-1; ZFHX1B; HSPC082; SMADIP1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot, ELISA
• Purity: Peptide Column Affinity Chromatography
• Synonyms: ZEB2; Polyclonal Antibody; Rabbit Polyclonal to Human ZEB2; ZEB2 | KIAA0569 | SIP1 | SMADIP1 | ZFX1B | SIP-1 | SMAD interacting protein 1 | Smad-interacting protein 1 | ZFHX1B | Zinc finger homeobox 1b | HSPC082; anti-ZEB2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ZEB2 antibody is predicted to not cross-react with ZEB1.
• Purity/Purification: Peptide Column Affinity Chromatography
• Form/Format: PBS, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1214

• Applicable Applications for anti-ZEB2 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), ELISA (EIA)
• Application Notes: IHC - Paraffin (5ug/ml); Western blot (1-2ug/ml); ; ZEB2 antibody can be used for detection of ZEB2 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunocytochemistry starting at 20 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 134 kDa Observed: 135 kDa.
• Target: Human ZEB2 / SIP-1
• Immunogen Species: ZEB/SIP antibody was raised against human
• Antigen Type: Synthetic peptide
• Immunogen: ZEB2/SIP-1 antibody was raised against an 18 amino acids synthetic peptide near the C-terminus of human ZEB2. The immunogen is located within the last 50 amino acids of ZEB2.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-ZEB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ZEB2 antibody IHC of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 ug/ml.)

Immunocytochemistry (ICC)

(Immunocytochemistry of ZEB2 in Jurkat cells with ZEB2 antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot analysis of ZEB2 in Jurkat cell lysate with ZEB2 antibody at (A) 1 and (B) 2ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 7662184
• NCBI GeneID: 9839
• NCBI Accession #: NP_055610.1
• NCBI GenBank Nucleotide #: NM_014795.3
• UniProt Accession #: O60315; Q9UED1; A0JP09; B7Z2P2; F5H814
• NCBI Official Full Name: zinc finger E-box-binding homeobox 2 isoform 1
• NCBI Official Synonym Full Names: zinc finger E-box binding homeobox 2
• NCBI Official Symbol: ZEB2
• NCBI Official Synonym Symbols: SIP1; SIP-1; ZFHX1B; HSPC082; SMADIP1
• NCBI Protein Information: zinc finger E-box-binding homeobox 2; zinc finger homeobox 1b; SMAD interacting protein 1; Smad-interacting protein 1
• UniProt Protein Name: Zinc finger E-box-binding homeobox 2
• Protein Family: Zinc finger E-box-binding homeobox
• UniProt Gene Name: ZEB2
• UniProt Synonym Gene Names: KIAA0569; SIP1; ZFHX1B; ZFX1B; SMADIP1
• UniProt Entry Name: ZEB2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jan 2010]

Uniprot Description

ZEB2: Transcriptional inhibitor that binds to DNA sequence 5'- CACCT-3' in different promoters. Represses transcription of E- cadherin. Defects in ZEB2 are the cause of Mowat-Wilson syndrome (MWIS); also known as Hirschsprung disease-mental retardation syndrome. A complex developmental disorder characterized by mental retardation, delayed motor development, epilepsy, microcephaly and a wide spectrum of clinically heterogeneous features suggestive of neurocristopathies at the cephalic, cardiac, and vagal levels. Some patients manifest Hirschsprung disease. Affected patients show an easily recognizable facial appearance with deep set eyes and hypertelorism, medially divergent, broad eyebrows, prominent columella, pointed chin and uplifted, notched ear lobes. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.

Protein type: DNA-binding; Motility/polarity/chemotaxis; Transcription, coactivator/corepressor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 2q22.3

Cellular Component: nucleus

Molecular Function: DNA binding; phosphatase regulator activity; metal ion binding

Biological Process: positive regulation of JNK activity; nervous system development; somitogenesis; transcription, DNA-dependent; cell proliferation in forebrain; hippocampus development; pigmentation during development; positive regulation of melanin biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; positive regulation of melanocyte differentiation; neural tube closure; positive regulation of transcription from RNA polymerase II promoter; neural crest cell migration; positive regulation of Wnt receptor signaling pathway

Disease: Mowat-wilson Syndrome