Rabbit anti-Human, Mouse NEK2 Polyclonal Antibody | anti-NEK2 antibody

NEK2 Polyclonal Antibody

Cat. Num. AAA3011148

• Gene Names: NEK2; NLK1; RP67; NEK2A; HsPK21; PPP1R111
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: NEK2; Polyclonal Antibody; NEK2 Polyclonal Antibody; HSPK 21; HsPK21; NEK 2; NEK2_HUMAN; NEK2A; Never in mitosis A-related kinase 2; NIMA (never in mitosis gene a) related kinase 2; NimA like protein kinase 1; NIMA related kinase 2; NimA related protein kinase 2; NimA-like protein kinase 1; NimA-related protein kinase 2; NLK 1; NLK1; Serine/threonine-protein kinase Nek2; anti-NEK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NEK2 polyclonal antibody detects endogenous levels of NEK2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 445

• Applicable Applications for anti-NEK2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human NEK2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of NEK2 polyclonal antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analysis of NEK2 polyclonal antibody in paraffin-embedded Human Kidney Cancer tissue.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells, using NEK2 polyclonal antibody)

Related Product Information for anti-NEK2 antibody

This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase.

NCBI and Uniprot Product Information

• NCBI GI #: 4505373
• NCBI GeneID: 4751
• NCBI Accession #: NP_002488.1
• NCBI GenBank Nucleotide #: NP_002488.1
• UniProt Accession #: P51955
• Molecular Weight: ~ 53kDa
• NCBI Official Full Name: serine/threonine-protein kinase Nek2 isoform 1
• NCBI Official Synonym Full Names: NIMA related kinase 2
• NCBI Official Symbol: NEK2
• NCBI Official Synonym Symbols: NLK1; RP67; NEK2A; HsPK21; PPP1R111
• NCBI Protein Information: serine/threonine-protein kinase Nek2
• UniProt Protein Name: Serine/threonine-protein kinase Nek2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: NEK2
• UniProt Synonym Gene Names: NEK2A; NLK1; NimA-related protein kinase 2
• UniProt Entry Name: NEK2_HUMAN

NCBI Description

This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

NEK2: a protein kinase of the NEK family. Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Phosphorylation of Hec1 by Nek2 is essential for faithful chromosome segregation

Protein type: Protein kinase, Other; Kinase, protein; EC 2.7.11.1; Nucleolus; Protein kinase, Ser/Thr (non-receptor); Other group; NEK family

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: kinetochore; spindle pole; centrosome; microtubule; intercellular bridge; condensed nuclear chromosome; protein complex; cytoplasm; nucleolus; midbody; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; protein phosphatase binding; ATP binding; protein kinase activity

Biological Process: mitosis; blastocyst development; regulation of attachment of spindle microtubules to kinetochore; mitotic sister chromatid segregation; organelle organization and biogenesis; protein amino acid autophosphorylation; regulation of mitotic centrosome separation; centrosome separation; spindle assembly; protein amino acid phosphorylation; negative regulation of DNA binding; chromosome segregation; meiotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; regulation of mitosis; mitotic cell cycle; G2/M transition of mitotic cell cycle

Disease: Retinitis Pigmentosa 67