Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NEK2 antibody.)
Rabbit NEK2 Polyclonal Antibody | anti-NEK2 antibody
NEK2 Antibody
Immunohistochemistry: 1:50 - 1:200
Immunofluorescence: 1:50 - 1:200
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NEK2 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NEK2 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney cancer using NEK2 antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney cancer using NEK2 antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human thyroid cancer using NEK2 antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human thyroid cancer using NEK2 antibody at dilution of 1:200 (400x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cell using NEK2 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cell using NEK2 antibody. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]
Uniprot Description
NEK2: a protein kinase of the NEK family. Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Phosphorylation of Hec1 by Nek2 is essential for faithful chromosome segregation
Protein type: Kinase, protein; Protein kinase, Other; Nucleolus; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; NEK family
Chromosomal Location of Human Ortholog: 1q32.3
Cellular Component: kinetochore; spindle pole; microtubule; centrosome; intercellular bridge; condensed nuclear chromosome; protein complex; cytoplasm; nucleolus; midbody; nucleus; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; protein phosphatase binding; ATP binding; protein kinase activity
Biological Process: mitosis; regulation of attachment of spindle microtubules to kinetochore; blastocyst development; mitotic sister chromatid segregation; organelle organization and biogenesis; regulation of mitotic centrosome separation; protein amino acid autophosphorylation; centrosome separation; spindle assembly; protein amino acid phosphorylation; negative regulation of DNA binding; chromosome segregation; meiotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; regulation of mitosis; mitotic cell cycle; G2/M transition of mitotic cell cycle
Disease: Retinitis Pigmentosa 67
Research Articles on NEK2
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Product Notes
The NEK2 nek2 (Catalog #AAA9401286) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NEK2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NEK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200. Researchers should empirically determine the suitability of the NEK2 nek2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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