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Western Blot (WB) (Western Blot analysis of extracts of Mouse thymus tissue lysate, using Cytokeratin 15 antibody.)

Rabbit anti-Human, Mouse Cytokeratin 15 Polyclonal Antibody | anti-KRT15 antibody

Cytokeratin 15 Polyclonal Antibody

Gene Names
KRT15; K15; CK15; K1CO
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Cytokeratin 15; Polyclonal Antibody; Cytokeratin 15 Polyclonal Antibody; Keratin; type I cytoskeletal 15; Cytokeratin-15; Short name=CK-15; Keratin-15; Short name=K15; KRT15; KRTB; Keratin 15; CK15; CK 15; Keratin15; anti-KRT15 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Cytokeratin 15 polyclonal antibody detects endogenous levels of Cytokeratin 15 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
456
Applicable Applications for anti-KRT15 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Immunogen
Recombinant full length Human Cytokeratin 15.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of Mouse thymus tissue lysate, using Cytokeratin 15 antibody.)

Western Blot (WB) (Western Blot analysis of extracts of Mouse thymus tissue lysate, using Cytokeratin 15 antibody.)
Related Product Information for anti-KRT15 antibody
Cytokeratin 15 is an intermediate filament (IF) type I protein that is responsible for the mechanical integrity of epithelial cells. Keratin family members are subdivided into cytokeratins and hair keratins. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for identification between basal cell carcinoma and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceus. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a identification difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 49kDa
NCBI Official Full Name
keratin, type I cytoskeletal 15
NCBI Official Synonym Full Names
keratin 15
NCBI Official Symbol
KRT15
NCBI Official Synonym Symbols
K15; CK15; K1CO
NCBI Protein Information
keratin, type I cytoskeletal 15
UniProt Protein Name
Keratin, type I cytoskeletal 15
Protein Family
UniProt Gene Name
KRT15
UniProt Synonym Gene Names
KRTB; CK-15; K15
UniProt Entry Name
K1C15_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2. [provided by RefSeq, Jul 2008]

Uniprot Description

K15: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: intermediate filament; nucleus

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: epidermis development

Research Articles on KRT15

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Product Notes

The KRT15 krt15 (Catalog #AAA3011011) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cytokeratin 15 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the KRT15 krt15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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